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Mass tag PCR for mutliplex diagnostics

  • US 20060003352A1
  • Filed: 04/28/2005
  • Published: 01/05/2006
  • Est. Priority Date: 04/29/2004
  • Status: Abandoned Application
First Claim
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1. A method for simultaneously detecting in a sample the presence of one or more of a plurality of different target nucleic acids comprising the steps of:

  • (a) contacting the sample with a plurality of nucleic acid primers simultaneously and under conditions permitting, and for a time sufficient for, primer extension to occur, wherein (i) for each target nucleic acid at least one predetermined primer is used which is specific for that target nucleic acid, (ii) each primer has a mass tag of predetermined size bound thereto via a labile bond, and (iii) the mass tag bound to any primer specific for one target nucleic acid has a different mass than the mass tag bound to any primer specific for any other target nucleic acid;

    (b) separating any unextended primers from any extended primers;

    (c) simultaneously cleaving the mass tags from any extended primers; and

    (d) simultaneously determining the presence and sizes of any mass tags so cleaved, wherein the presence of a cleaved mass tag having the same size as a mass tag of predetermined size previously bound to a predetermined primer indicates the presence in the sample of the target nucleic acid specifically recognized by that predetermined primer.

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