Compositions and methods for analyte detection
First Claim
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1. A method of detecting a target analyte in a test sample comprising:
- a) adding said sample to a solution comprising a compound comprising;
i) a solvent accessible transition metal complex with a first redox potential;
ii) a linker comprising an alkyl chain terminating in a pyridine; and
iii) a binding ligand that binds said target analyte;
wherein said solvent accessible transition metal complex is covalently attached to said binding ligand by said linker;
such that upon binding of said analyte to said binding ligand, a solvent inhibited transition metal complex is formed with a second redox potential; and
b) detecting said second redox potential as an indication of the presence of said target analyte.
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Abstract
The invention relates to novel methods and compositions for the detection of analytes using the nuclear reorganization energy, λ, of an electron transfer process. In particular, the present invention provides probes of the outer-sphere environment of a redox center and methods of correlating changes in electrochemical properties to characterize the same.
21 Citations
12 Claims
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1. A method of detecting a target analyte in a test sample comprising:
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a) adding said sample to a solution comprising a compound comprising;
i) a solvent accessible transition metal complex with a first redox potential;
ii) a linker comprising an alkyl chain terminating in a pyridine; and
iii) a binding ligand that binds said target analyte;
wherein said solvent accessible transition metal complex is covalently attached to said binding ligand by said linker;
such that upon binding of said analyte to said binding ligand, a solvent inhibited transition metal complex is formed with a second redox potential; and
b) detecting said second redox potential as an indication of the presence of said target analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification