Cell display libraries

• US 20060003387A1
• Filed: 02/07/2005
• Published: 01/05/2006
• Est. Priority Date: 02/05/2004
• Status: Active Grant

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Belcher, Angela M., Peelle, Beau R., Wittrup, Karl Dane, Krauland, Eric
• Granted Patent: US 8,450,247 B2
• Time in Patent Office: Days
• US Class Current: 435/7.200
• CPC Class Codes: C12N 15/1037 Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
• CPC Class Codes: G01N 33/569 for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

Eukaryotic cell display libraries for use in panning processes comprising expressed biomolecules for specific and selective binding and enrichment to solid material surfaces including, for example, metal, magnetic, and semiconducting surfaces. Display can be regulated. Peptide and protein display on yeast cells are preferred. Solid materials can be fabricated in the presence of cell display libraries which have been subjected to panning against the solid materials. Nanoparticles can be grown in the presence of the biomolecules from reactive precursors. The nanoparticles can show quantum confinement effects. Self-healing films can be prepared.

Claims

1. A eukaryotic cell composition comprising a plurality of eukaryotic cells which selectively bind to a solid material having a surface.

2. The composition according to claim 1, wherein the eukaryotic cells are yeast, insect, plant, or mammalian cells.

3. The composition according to claim 1, wherein the eukaryotic cells are yeast cells which comprise biomolecules which selectively bind to a solid material having a surface.

4. The composition according to claim 1, wherein the eukaryotic cells are yeast cells which comprise peptide sequences which selectively bind to a solid material having a surface.

5. The composition according to claim 1, wherein the solid material having a surface is a crystalline material, an inorganic material, a semiconductor material, a metallic material, a magnetic material, a ceramic material, an organic material, or a polymer material having a surface.

6. A eukaryotic cell composition consisting essentially of eukaryotic cells which specifically bind to a solid material having a surface and eukaryotic cells which do not specifically bind to the solid material having a surface.

7. The composition according to claim 6, wherein the eukaryotic cells which specifically bind are yeast, insect, plant, or mammalian cells.

8. The composition according to claim 6, wherein the eukaryotic cells which specifically bind consist essentially of biomolecules which specifically bind to a solid material having a surface.

9. The composition according to claim 6, wherein the eukaryotic cells are yeast cells and the solid material having a surface is a crystalline material, an inorganic material, a semiconductor material, a metallic material, a magnetic material, a ceramic material, an organic material, or a polymer material having a surface.

10. A host eukaryotic cell comprising one or more biomolecules which selectively bind with a solid material having a surface.

11. The cell according to claim 10, wherein the one or more biomolecules comprise peptide or protein.

12. The cell according to claim 10, wherein the solid material having a surface is an inorganic or organic material.

13. The cell according to claim 10, wherein the cell is a mammalian or yeast cell, and the solid material having a surface is a crystalline material, an inorganic material, a semiconductor material, a metallic material, a magnetic material, a ceramic material, an organic material, or a polymer material having a surface.

14. A cell covered material comprising one or more eukaryotic cells which are selectively bound to a solid material having a surface.

15. The material according to claim 14, wherein the eukaryotic cells are yeast, insect, plant, or mammalian cells.

16. The material according to claim 14, wherein the eukaryotic cells are yeast cells which comprise biomolecules which are selectively bound to a solid material having a surface.

17. The material according to claim 14, wherein the solid material having a surface is a crystalline material, an inorganic material, a semiconductor material, a metallic material, a magnetic material, a ceramic material, an organic material, or a polymer material having a surface.

18. The material according to claim 14, wherein the cell-covered material is a self-healing cell-covered material.

19. A method for selective binding of biomolecules from a cell display library to a solid material surface comprising the steps of:

providing a eukaryotic combinatorial cell display library, wherein the library comprises a plurality of expressed biomolecules,

providing a solid material having a surface;

contacting the cell display library with the solid material having a surface under conditions which result in selective binding of the plurality of expressed biomolecules from the eukaryotic cell display library to the surface.

20. The method of claim 19, wherein the combinatorial cell display library is a yeast, insect, plant, or mammalian cell display library.

21. The method according to claim 19, wherein the plurality of biomolecules is a plurality of proteins or peptides.

22. The method according to claim 19, wherein the solid material having a surface is a crystalline solid material, inorganic solid material, semiconductor material, metallic material, magnetic material, ceramic material, organic material, or a polymer material having a surface.

23. The method according to claim 19, further comprising the step of regulating the expression of the library.

24. The method according to claim 19, further comprising the step of isolating expressed biomolecules which selectively bind to the solid material having a surface.

25. A method of growing particulate solid material comprising the steps of:

mixing one or more precursor reagents for the solid particulate material with one or more eukaryotic cell combinatorial display library members selected for specific binding to the solid particulate material, under conditions wherein the solid particulate material is formed in the presence of the one or more eukaryotic combinatorial display library members.

26. The method according to claim 25, wherein the solid particulate material is nanoparticulate material.

27. The method according to claim 25, wherein the solid particulate material is inorganic, organic, magnetic, semiconductor, or metallic particulate material.

28. A method of growing particulate solid material comprising the steps of:

identifying a biomolecule which selectively binds to a solid material from a eukaryotic cell display library,

mixing one or more precursor reagents for the solid material with the biomolecule under conditions wherein the solid material is formed as a particulate solid material.

29. The method according to claim 28, wherein the eukaryotic cell display library is a yeast or mammalian cell display library, and the biomolecule is a peptide or protein.

30. The method according to claim 28, wherein the solid material is an inorganic material.