Measurement method using biosensor

• US 20060003395A1
• Filed: 05/26/2005
• Published: 01/05/2006
• Est. Priority Date: 05/26/2004
• Status: Active Grant

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Hayashi, Katsumi, Yamamoto, Masayoshi, Ezoe, Toshihide, Ogura, Nobuhiko
• Granted Patent: US 7,470,549 B2
• Time in Patent Office: Days
• US Class Current: 435/7.920
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

It is an object of the present invention to provide a method for simultaneously carrying out the detection or measurement of a substance interacting with a physiologically active substance immobilized on a biosensor and the analysis of the effects of the above substance on the biological activity of the physiologically active substance. The present invention provides a measurement method using a biosensor formed by immobilizing a physiologically active substance on a substrate, wherein the detection and measurement of a substance interacting with said physiologically active substance and the measurement of the biological activity of said physiologically active substance are carried out on said single biosensor.

Claims

1. A measurement method using a biosensor formed by immobilizing a physiologically active substance on a substrate, wherein the detection and measurement of a substance interacting with said physiologically active substance and the measurement of the biological activity of said physiologically active substance are carried out on said single biosensor.

2. The measurement method according to claim 1, wherein the physiologically active substance is a protein.

3. The measurement method according to claim 1, wherein the physiologically active substance is an enzyme.

4. The measurement method according to claim 1, wherein the detection or measurement of a substance interacting with the physiologically active substance is carried out by surface plasmon resonance analysis.

5. The measurement method according to claim 4, wherein the surface plasmon resonance analysis is carried out by using a biosensor used for a surface plasmon resonance measurement device comprising:

a dielectric block;

a metal film formed on a face of the dielectric block;

a light source for generating a light beam;

an optical system for allowing said light beam to enter said dielectric block such that total reflection conditions can be obtained at the interface between said dielectric block and said metal film and that components at various incident angles can be contained; and

a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of the light beam totally reflected at said interface,

said biosensor being comprised of said dielectric block and said metal film, wherein said dielectric block is formed as one block comprising the entirety of the entrance face and exit face of said light beam and a face on which said metal film is formed, said metal film is integrated with the dielectric block.

6. The measurement method according to claim 1, wherein the measurement of the biological activity of the physiologically active substance is carried out by spectroscopic measurement.

7. The measurement method according to claim 1, which comprises:

(1) a step of allowing a test substance to come into contact with a biosensor formed by immobilizing a physiologically active substance on a substrate;

(2) a step of detecting or measuring the interaction between the physiologically active substance and the test substance; and

(3) a step of measuring the biological activity of the physiologically active substance in the presence of the test substance.

8. A method for screening a test substance, which comprises:

(1) a step of allowing a test substance to come into contact with a biosensor formed by immobilizing a physiologically active substance on a substrate, so as to detect the test substance interacting with said physiologically active substance by surface plasmon resonance analysis or local plasmon resonance analysis; and

(2) a step of measuring the biological activity of said physiologically active substance in the presence of said test substance.

9. The screening method according to claim 8, wherein 1 to 10 molecules of the test substances bind to a single molecule of the physiologically active substance.

10. The screening method according to claim 8, wherein the detection of a plurality of test substances by surface plasmon resonance analysis or local plasmon resonance analysis is carried out in a parallel detection approach.

11. The screening method according to claim 8, wherein the immobilization of a physiologically active substance on a biosensor and the detection of a test substance interacting with said physiologically active substance is carried out at different positions.

12. The screening method according to claim 8, wherein a test substance interacting with the physiologically active substance is detected by surface plasmon resonance analysis or local plasmon resonance analysis based on any of a binding amount, a binding constant, a binding rate constant, or a dissociation rate constant.

13. The screening method according to claim 8, wherein the biological activity of the physiologically active substance is indicated as any of a receptor activity level, an enzyme activity level, an antibody activity level, a metabolism activity level, a membrane potential, an ion emission level, or a gene expression level.

14. The screening method according to any of claim 8, wherein virtual screening is carried out on test substances before step (1), so as to reduce the number of test substances to be subjected to screening.

15. The screening method according to claim 8, wherein screening is automatically carried out using a screening device to which a standard value has previously been inputted.