Cell culture scaffold, methods of manufacturing the scaffold and cell culture methods

• US 20060003442A1
• Filed: 02/04/2005
• Published: 01/05/2006
• Est. Priority Date: 06/30/2004
• Status: Active Grant

• Current Assignee: Hokkaido University
• Original Assignee: Hokkaido University
• Inventors: Osada, Yoshihito, Gong, Jian Ping, Chen, Yongmei
• Granted Patent: US 7,435,594 B2
• Time in Patent Office: Days
• US Class Current: 435/325
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12N 2533/30 Synthetic polymers
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

A cell culture scaffold containing a gel having a network structure comprised of a synthetic polymer such that cultured cells spread in a shorter time and the number of adsorbed cultured cells per unit area is larger than in the case of using a gel having a network structure comprised of polyacrylic acid, while taking advantage of synthetic polymers with low manufacturing cost, easy quality control and no risk of virus infection in cultured cell. Used as the cell culture scaffold is a gel containing asynthetic polymer obtained by polymerization or copolymerization of a monomer having a sulfonic group such as p-styrenesulfonic acid sodium salt (NaSS) and 2-acrylamide-2-methylpropane sulfonic acid sodium salt (NaAMPS).

Claims

1. A cell culture scaffold containing a gel having a network structure comprising a synthetic polymer obtained by polymerization or copolymerization of a monomer having a sulfonic group.

2. The cell culture scaffold according to claim 1, wherein the monomer having a sulfonic group is an aromatic compound.

3. The cell culture scaffold according to claim 1, wherein the monomer having a sulfonic group is a p-styrenesulfonic acid alkali metal salt or a 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt.

4. The cell culture scaffold according to claim 1, wherein a molar fraction of the p-styrenesulfonic acid alkali metal salt as a structural unit is 6 mol % or more in the synthetic polymer.

5. The cell culture scaffold according to claim 1, wherein a molar fraction of the 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt as a structural unit is 30 mol % or more in the synthetic polymer.

6. The cell culture scaffold according to claim 3, wherein a degree of crosslinking ranges from 0.1 mol % to 10 mol % in the synthetic polymer.

7. The cell culture scaffold according to claim 1, wherein the network structure comprising the synthetic polymer is present on the uppermost layer of the surface of the gel.

11. A cell culture method using the cell culture scaffold according to claim 1.

8. A cell culture scaffold containing a gel having an interpenetrating polymer network structure in which a network structure comprising a synthetic polymer obtained by polymerization or copolymerization of a p-styrenesulfonic acid alkali metal salt or a 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt penetrates a network structure comprising a polymer having a crosslinking structure, or a semi-interpenetrating polymer network structure in which the synthetic polymer is a linear polymer and the linear synthetic polymer penetrates the network structure with the crosslinking structure.

9. The cell culture scaffold according to claim 8, wherein the network structure comprising the polymer having the crosslinking structure is bacteria cellulose.

10. A method of manufacturing a cell culture scaffold, wherein in generating a gel having a network structure comprising a synthetic polymer obtained by copolymerization of a monomer having a sulfonic group, surface potential of the gel is adjusted by adjusting a molar fraction of another monomer subjected to the copolymerization relative to the monomer having the sulfonic group.