Novel polynucleotides related to oligonucleotide arrays to monitor gene expression
First Claim
1. An isolated nucleic acid molecule having a polynucleotide sequence selected from the group consisting of the polynucleotide sequences of SEQ ID NOs:
- 3421-3574, complements thereof, and subsequences thereof.
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Abstract
The present invention provides an oligonucleotide array capable of identifying genes and related pathways involved with the induction of a particular phenotype by a cell line, e.g., the genes and related pathways involved with the induction of transgene expression by the cell line. The invention is particularly useful when there is little or no information about the genome of the cell line being studied, because it provides methods for identifying consensus sequences for known and previously undiscovered genes, and for designing oligonucleotide probes to the identified consensus sequences. Additionally, when the array is to be used to determine optimal conditions for expression of a transgene by the cell line, the invention teaches methods of including oligonucleotide probes to transgene sequences in the array. The invention also provides methods of using the array to identify genes and related pathways involved with the induction of a particular cell line phenotype. The invention also provides novel polynucleotides of undiscovered genes (i.e., a gene that had not been sequenced and/or shown to be expressed by CHO cells) and novel polynucleotides involved with the induction of a particular cell phenotype, e.g., increased survival when grown under stressful culture conditions, increased transgene expression, decreased production of an antigen, etc. These novel polynucleotides are termed novel CHO sequences and differential CHO sequences, respectively. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention. The invention further provides methods of using the polynucleotides of the invention.
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Citations
19 Claims
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1. An isolated nucleic acid molecule having a polynucleotide sequence selected from the group consisting of the polynucleotide sequences of SEQ ID NOs:
- 3421-3574, complements thereof, and subsequences thereof.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- 10. A method of increasing transgene expression by a cell population comprising targeting the cell population with an inhibitory polynucleotide.
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15. A method of identifying a compound capable of increasing survival of a cell population grown under stressful conditions, increasing transgene expression by a cell population, or decreasing the production of an antigen comprising the steps of:
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(a) contacting a first cell population expressing a gene having a polynucleotide sequence selected from the group consisting of the polynucleotide sequences of SEQ ID NOs;
3421-3572, complements thereof, and subsequences thereof with one of a plurality of test compounds; and
(b) comparing the expression of the gene having a polynucleotide sequence selected from the group consisting of the polynucleotide sequences of SEQ ID NOs;
3421-3572, complements thereof, and subsequences thereof in the first population of cells with the expression of the gene in a second population of cells not contacted with the test compound, wherein a decrease in the expression of the gene having a polynucleotide sequence selected from the group consisting of the polynucleotide sequences of SEQ ID NOs;
3421-3572, complements thereof, and subsequences thereof in the first sample, as compared with that in the second sample, identifies the compound as capable of increasing transgene expression. - View Dependent Claims (16)
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17. A novel CHO polynucleotide having a polynucleotide sequence selected from the group consisting of the polynucleotide sequences of SEQ ID NOs:
- 19-3573, wherein the polynucleotide sequence was previously undiscovered.
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18. A differential CHO polynucleotide having a polynucleotide sequence selected from the group consisting of the polynucleotide sequences of SEQ ID NOs:
- 19-3574, wherein expression of the differential CHO polynucleotide is correlated with increased survival of a cell grown under stressful culture conditions, increased transgene expression, or decreased production of an antigen.
- View Dependent Claims (19)
Specification