Mesenchymal precursor cell

• US 20060008452A1
• Filed: 07/11/2005
• Published: 01/12/2006
• Est. Priority Date: 07/07/1999
• Status: Active Grant

• Current Assignee: Mesoblast, Inc. (Mesoblast Ltd.)
• Original Assignee: Medvet Science Pty. Ltd.
• Inventors: Gronthos, Stan, Zannettino, Andrew, Simmons, Paul
• Granted Patent: US 7,399,632 B2
• Time in Patent Office: Days
• US Class Current: 424/93.210
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: C12N 2500/42 Organic phosphate, e.g. beta glycerophosphate
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 5/0663 Bone marrow mesenchymal stem cells (BM-MSC)

Abstract

A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.

Claims

1-51. -51. (canceled)

52. A method of enriching mesenchymal precursor cells, the method comprising selecting cells which are STRO-1^bright, VACM-1^bright, CD146^bright and/or STRO-2^bright.

77. A method of enriching mesenchymal precursor cells, the method comprising the step of enriching for cells based on at least two markers, said markers being either:

a) the presence of markers specific for mesenchymal precursor cells, or

b) the absence of markers specific for differentiated mesenchymal precursor cells, or

c) the levels of expression of markers specific for differentiated mesenchymal precursor cells.