Genotype specific detection of Chlamydophila psittaci
First Claim
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1. An ex vivo or in vitro method for the identification of the presence of DNA of a genotype of Cp. psittaci in a sample, said method comprising the steps of:
- incubating said sample with a first oligonucleotide which is capable of specifically hybridising to DNA of a genotype of Cp. psittaci, and, determining the binding of said first oligonucleotide to DNA within said sample, which binding is indicative of the presence of DNA of a genotype of Cp. psittaci in said sample.
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Abstract
The present invention describes novel methods for the specific detection and identification of Chlamydophila psittaci genotypes. According to one embodiment the method makes use of quantitative PCR with internal probes and optionally competitor probes which increase specificity. The invention also describes a strain of Cp. psittaci with a novel genotype EB and methods to distinguish said novel genotype from previously identified genotypes.
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28 Claims
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1. An ex vivo or in vitro method for the identification of the presence of DNA of a genotype of Cp. psittaci in a sample, said method comprising the steps of:
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incubating said sample with a first oligonucleotide which is capable of specifically hybridising to DNA of a genotype of Cp. psittaci, and, determining the binding of said first oligonucleotide to DNA within said sample, which binding is indicative of the presence of DNA of a genotype of Cp. psittaci in said sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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- 18. A diagnostic kit for the detection of a Cp. psittaci genotype comprising one or more oligonucleotides capable of hybridizing specifically to a sequence within the DNA of a genotype of Cp. psittaci.
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24. A strain of a Cp. psittaci bacterium, designated as Cp. psittaci genotype EB, said strain characterized in that it comprises the OmpA sequence depicted in SEQ ID NO:
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25. A method of generating oligonucleotide sequences useful for the discrimination between at least two genotypes of Cp. psittaci, said method comprising the steps of:
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a) providing a multiple alignment of a part of the genomic sequence of said at least two Cp. psittaci genotypes, b) identifying regions which contain sequence differences within said part of the genomic sequence, c) synthesizing one or more oligonucleotides comprising a sequence wherein said sequences differences occur. - View Dependent Claims (26, 27, 28)
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Specification