Plant transcriptional regulators of abiotic stress
First Claim
1. A recombinant polynucleotide comprising a nucleotide sequence that hybridizes over its full length to SEQ ID NO:
- 1 or its complement under stringent conditions that include two wash steps of 6×
SSC at 65°
C., each step being 10-30 minutes in duration.
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Accused Products
Abstract
The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, variants of naturally-occurring sequences, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties, including improved cold and other osmotic stress tolerance, as compared to wild-type or reference plants. The invention also pertains to expression systems that may be used to regulate these transcription factor polynucleotides, providing constitutive, transient, inducible and tissue-specific regulation.
72 Citations
36 Claims
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1. A recombinant polynucleotide comprising a nucleotide sequence that hybridizes over its full length to SEQ ID NO:
- 1 or its complement under stringent conditions that include two wash steps of 6×
SSC at 65°
C., each step being 10-30 minutes in duration. - View Dependent Claims (2, 3, 4, 5, 6, 21, 22, 23, 24, 25)
- 1 or its complement under stringent conditions that include two wash steps of 6×
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7. A transgenic plant having increased abiotic stress tolerance, wherein said transgenic plant comprises a recombinant polynucleotide comprising a nucleotide sequence that encodes a member of the G867 clade of transcription factor polypeptides;
wherein the member of the G867 clade of transcription factor polypeptides comprises an AP2 domain, a DML motif, and a B3 domain, in order from N-terminal to C-terminal. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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26. A method for producing a transformed plant with greater tolerance to an abiotic stress than a control plant, the method comprising:
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(a) providing an expression vector comprising a polynucleotide sequence encoding a member of the G867 clade of transcription factor polypeptides;
wherein the member of the G867 clade of transcription factor polypeptides comprises an AP2 domain, a DML motif, and a B3 domain, in order from N-terminal to C-terminal; and
wherein the polynucleotide sequence is operably linked to an inducible or tissue specific promoter that controls expression of the polynucleotide sequence;
(b) transforming a target plant with the expression vector to produce the transformed plant; and
(c) growing the transformed plant comprising the expression vector. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
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Specification