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Monomeric red fluorescent proteins

  • US 20060008878A1
  • Filed: 04/07/2005
  • Published: 01/12/2006
  • Est. Priority Date: 04/07/2004
  • Status: Active Grant
First Claim
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8. An isolated polynucleotide encoding a variant polypeptide of the rapidly maturing Discosoma red fluorescent protein T4 (DsRed.T4) of SEQ ID NO:

  • 3, the variant polypeptide exhibiting reduced oligomerization relative to DsRed.T4 and exhibiting detectable red fluorescence, the variant polypeptide comprising;

    at least one amino substitution selected from the group consisting of K83M, K83L, K163Q, K163M, K163H, and Y193H;

    at least three amino acid substitutions selected from the group consisting of E26Y, K92T, V96S, T106E, T108Q, 1125K, S131A, 1180V, and M182K; and

    at least three amino acid substitutions selected from the group consisting of R149K, R153Q, H162S, L174T, E176D, Y192N, R216H, H222S, L223G, and F224S.

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