Monomeric red fluorescent proteins
First Claim
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8. An isolated polynucleotide encoding a variant polypeptide of the rapidly maturing Discosoma red fluorescent protein T4 (DsRed.T4) of SEQ ID NO:
- 3, the variant polypeptide exhibiting reduced oligomerization relative to DsRed.T4 and exhibiting detectable red fluorescence, the variant polypeptide comprising;
at least one amino substitution selected from the group consisting of K83M, K83L, K163Q, K163M, K163H, and Y193H;
at least three amino acid substitutions selected from the group consisting of E26Y, K92T, V96S, T106E, T108Q, 1125K, S131A, 1180V, and M182K; and
at least three amino acid substitutions selected from the group consisting of R149K, R153Q, H162S, L174T, E176D, Y192N, R216H, H222S, L223G, and F224S.
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Abstract
Disclosed are sequences encoding monomeric variants of DsRed fluorescent proteins and methods of use.
72 Citations
28 Claims
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8. An isolated polynucleotide encoding a variant polypeptide of the rapidly maturing Discosoma red fluorescent protein T4 (DsRed.T4) of SEQ ID NO:
- 3, the variant polypeptide exhibiting reduced oligomerization relative to DsRed.T4 and exhibiting detectable red fluorescence, the variant polypeptide comprising;
at least one amino substitution selected from the group consisting of K83M, K83L, K163Q, K163M, K163H, and Y193H;
at least three amino acid substitutions selected from the group consisting of E26Y, K92T, V96S, T106E, T108Q, 1125K, S131A, 1180V, and M182K; and
at least three amino acid substitutions selected from the group consisting of R149K, R153Q, H162S, L174T, E176D, Y192N, R216H, H222S, L223G, and F224S. - View Dependent Claims (1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
- 3, the variant polypeptide exhibiting reduced oligomerization relative to DsRed.T4 and exhibiting detectable red fluorescence, the variant polypeptide comprising;
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28-1. The method of claim 27, further comprising monitoring temporal or spatial changes in red fluorescence.
Specification