Nucleic acid detecting or quantifying processes
4 Assignments
0 Petitions
Accused Products
Abstract
This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
133 Citations
889 Claims
-
1. (canceled)
-
2-860. -860. (canceled)
-
861. A process for detecting or quantifying analytes of interest, said process comprising the steps of:
-
1) providing;
a) an array of solid surfaces comprising a plurality of discrete areas;
wherein at least two of said discrete areas comprise a chimeric composition comprising a nucleic acid portion; and
a non-nucleic acid portion;
wherein said nucleic acid portion of a first discrete area has the same sequence as the nucleic acid portion of a second discrete area; and
wherein said non-nucleic acid portion has a binding affinity for analytes of interest;
b) a sample containing or suspected of containing one or more of said analytes of interest; and
c) signal generating means;
2) contacting said array a) with the sample b) under conditions permissive of binding said analytes to said non-nucleic acid portion;
3) contacting said bound analytes with said signal generating means; and
4) detecting or quantifying the presence of said analytes. - View Dependent Claims (862, 863, 864, 865, 866, 867, 868, 869, 870, 871, 872, 873, 874)
-
-
875. A process for detecting or quantifying analytes of interest, said process comprising the steps of:
-
1) providing;
a) an array of solid surfaces comprising a plurality of discrete areas;
wherein at least two of said discrete areas comprise a chimeric composition comprising a nucleic acid portion; and
a non-nucleic acid portion;
wherein said nucleic acid portion of a first discrete area has the same sequence as the nucleic acid portion of a second discrete area; and
wherein said non-nucleic acid portion has a binding affinity for analytes of interest;
b) a sample containing or suspected of containing one or more of said analytes of interest; and
c) signal generating means;
2) labeling said analytes of interest with said signal generating means;
3) contacting said array a) with said labeled analytes under conditions permissive of binding said labeled analytes to said non-nucleic acid portion; and
4) detecting or quantifying the presence of said analytes. - View Dependent Claims (876, 877, 878, 879, 880, 881, 882, 883, 884, 885, 886, 887, 888)
-
-
889-952. -952. (canceled)
Specification