Analog probe complexes
First Claim
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1. A L-DNA/PNA analog probe complex comprising:
- a first segment comprising a protein nucleic acid (PNA) segment, wherein said PNA segment comprises a PNA probe sequence that is capable of binding to a target sequence;
a fluorescent marker attached to the first segment; and
a second segment comprising a left-handed DNA (L-DNA) sequence that is capable of dissociably binding to said PNA probe sequence.
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Abstract
The present invention relates to the detection of target sequences. The present description discloses compositions and methods involving analog nucleic acids, such as PNA and L-DNA, for the detection of nucleic acids. Additionally, hybrid detectable markers are provided.
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Citations
19 Claims
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1. A L-DNA/PNA analog probe complex comprising:
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a first segment comprising a protein nucleic acid (PNA) segment, wherein said PNA segment comprises a PNA probe sequence that is capable of binding to a target sequence;
a fluorescent marker attached to the first segment; and
a second segment comprising a left-handed DNA (L-DNA) sequence that is capable of dissociably binding to said PNA probe sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of in-situ hybridization comprising:
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contacting an analog probe complex with a sample comprising a target sequence, said analog probe complex comprising a) a first segment comprising a first PNA sequence, wherein said first PNA sequence will hybridize to a target sequence, b) a second segment, said second segment comprising a first L-DNA sequence, wherein said first L-DNA sequence can dissociably bind to said first PNA sequence, c) a fluorophore attached to the first segment, and d) a quencher attached to the second segment;
allowing the PNA sequence to bind to the target sequence;
fixing the sample; and
observing a fluorescence from the fluorophore. - View Dependent Claims (11, 12, 13, 14, 15)
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16. A kit for wash free in situ hybridization comprising:
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an analog probe complex comprising
1) a first section comprising a fluorescent marker and a PNA sequence, wherein said PNA sequence will hybridize to a target sequence and can dissociably bind to a L-DNA sequence, and
2) a second section comprising an L-DNA sequence and a quencher, wherein said L-DNA sequence can dissociably bind to said PNA sequence; and
a fixation reagent. - View Dependent Claims (17, 18)
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19. An analog probe complex for the detection of nucleic acids, said analog probe complex comprising:
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a first nucleic acid analog segment comprising a means for detecting a target sequence; and
a second nucleic acid analog segment comprising a means for detecting the second nucleic acid analog segment and a means for hybridizing said first segment with said second segment.
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Specification