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Transgenic non-human animals for producing heterologous and chimeric antibodies

  • US 20060015949A1
  • Filed: 12/10/2004
  • Published: 01/19/2006
  • Est. Priority Date: 08/29/1990
  • Status: Abandoned Application
First Claim
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1. A method for preparing a human antibody, which method comprises culturing a host cell comprising:

  • (i) a human immunoglobulin heavy chain recombinant construct comprising a nucleic acid molecule that encodes a human heavy chain variable region from a mouse B lymphocyte operably linked to a human heavy chain constant region, and (ii) a human immunoglobulin light chain recombinant construct comprising a nucleic acid molecule that encodes a human light chain variable region from a mouse B lymphocyte operably linked to a human light chain constant region, wherein the mouse B lymphocyte is from a transgenic mouse having an unrearranged human heavy chain immunoglobulin variable region comprising a plurality of human VH gene segments, a plurality of human D gene segments and a plurality of human JH gene segments, and having an unrearranged human light chain immunoglobulin variable region comprising a plurality of human VL gene segments and a plurality of human JL gene segments;

    under conditions that permit expression of the human heavy chain and human light chain to form an antibody.

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