Device and method for analyizing a sample

• US 20060018790A1
• Filed: 09/23/2005
• Published: 01/26/2006
• Est. Priority Date: 04/26/1996
• Status: Abandoned Application

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Koike, Masufumi, Naka, Michio, Hirayama, Kouji, Okuda, Hisashi, Higuchi, Yoshihiko
• Application Number:
  US11/234,455
• Publication Number:
  US 20060018790A1
• Time in Patent Office: Days
• US Class Current: 422/58
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/084 Passive control of flow resistance
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: G01N 1/14 Suction devices, e.g. pumps; Ejector devices
• CPC Class Codes: G01N 2021/0321 One time use cells, e.g. integrally moulded
• CPC Class Codes: G01N 2021/0325 Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/11 Filling or emptying of cuvettes
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72
• CPC Class Codes: G01N 33/521 Single-layer analytical elements

Abstract

This invention provides a device for analyzing a sample which is capable of performing rapid and precise analysis of a small amount of sample, and whose gradient in a measuring apparatus is not limited. This device comprises approximately a rectangular plate shaped body comprising a base member made of resin and a transparent covering. A first depressed cylindrical concave portion is formed in the upper surface of the base member, and a groove is formed in communication with the first depressed cylindrical concave portion, the groove extending to the end of the protrusion portion 5c, and a second depressed cylindrical concave portion which is smaller than the first depressed cylindrical concave portion is formed in a certain position in the groove, the end of the groove opening to the outside at the end of the protrusion portion 5c. Then, the transparent covering is placed over the upper surface of the base member and then integrated together. As a result, the first depressed cylindrical concave portion, the groove, the second depressed cylindrical concave portion, and the opening at the end of the groove are formed into a suction pressure generating chamber, a drawing channel, an analytical section, and an opening for drawing a sample, respectively.

Claims

1-27. -27. (canceled)

28. A device for collecting a sample for analysis, comprising:

a main body dimensioned to manipulated by hand;

a suction pressure generator comprising a chamber formed in the main body;

a drawing channel formed in the main body in communication with the chamber of the suction pressure generator, an opening in the main body being formed at the end of said drawing channel distal with respect to said suction pressure generator,

a flexible cover on the main body, whereby changes in pressure in the chamber of the suction pressure generator are created by movement of the flexible cover, and

an analytical section formed in said drawing channel between the suction generator and the opening, the analytical section communicating directly with the exterior of the device through the drawing channel,

wherein in use a sample is drawn into the main body through the opening by suction pressure developed by said suction pressure generator, and then the sample is transferred by the suction pressure through the drawing channel into the analytical section.

29. A device as claimed in claim 28 wherein the device has at least one dimension selected from:

an overall length of 15 to 100 mm;

a width of 20 to 50 mm;

a width of 5 to 20 mm;

or a thickness of 1 to 5 mm.

30. A device as claimed in claim 28, wherein the device is designed to be discarded after a single use.

31. A device as claimed in claim 30, wherein a liquid flow resistance in a first portion of the drawing channel between said analytical section and said suction pressure generator is greater than a liquid flow resistance in the bypass channel and a liquid flow resistance in a second portion of the drawing channel between said analytical section and a position at which said bypass channel branches.

32. A device as claimed in claim 28, wherein a positive pressure can be generated to return a sample withdrawn from the analytical section to the analytical section.

33. A device as claimed in claim 28, wherein the opening has a shape enlarging toward the end.

34. A device as claimed in claim 28, wherein a liquid pooling portion is formed between the opening and the drawing channel, and an air vent passage branches from a portion of the drawing channel between the liquid pooling portion and the analytical section, the end of the air vent passage opening to the outside.

35. A device as claimed in claim 34, wherein the liquid flow resistance in the air vent passage is larger than the liquid flow resistance in the liquid pooling portion.

36. A device as claimed in claim 28, wherein the analytical section formed in the drawing channel serves as a reagent positioning section and a reagent reaction section.

37. A device as claimed in claim 28, wherein a reagent positioning section, a reagent reaction section and an analytical section are provided independently in certain positions in the drawing channel.

38. A device as claimed in claim 37, wherein a plurality of reagent positioning section are provided in certain positions in the drawing channel.

39. A device as claimed in claim 28, wherein a concave portion with a cylindrical inner shape is formed in the main body as the chamber of the suction pressure generator and the flexible cover is disposed over the concave portion.

40. A device as claimed in claim 28, wherein the analytical section is wider than the drawing channel and the drawing channel extends from the analytical section to the suction pressure generator.