METHOD AND APPARATUS FOR NUCLEIC ACID SEQUENCING AND IDENTIFICATION
First Claim
1. A method comprising:
- a) placing a template nucleic acid into four chambers, each chamber being associated with different nanopores and each chamber to comprising a different of labeled nucleotide;
b) synthesizing a labeled nucleic acid from the template nucleic acid;
c) contacting the labeled nucleic acid in each chamber with a its associated nanopore, wherein the labeled nucleic acid passes through the associated nanopore;
d) detecting a labeled nucleotide;
e) compiling a nucleotide distance map for each type of labeled nucleotide; and
f) determining the sequence of the nucleic acid from the nucleotide distance maps.
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Abstract
The methods and apparatus 100 disclosed herein are of use for sequencing and/or identifying nucleic acids 230, 310. Nucleic acids 230, 310 containing labeled nucleotides 235, 245, 315 may be synthesized and passed through nanopores 255, 310. Detectors 257, 345 operably coupled to the nanopores 255, 310 may detect the labeled nucleotides 235, 245, 315. By determining the time intervals at which labeled nucleotides 235, 245, 315 are detected, distance maps 140 for each type of labeled nucleotide 235, 245, 315 may be compiled. The distance maps 140 in turn may be used to sequence 150 and/or identify 160 the nucleic acid 230, 310. In different embodiments of the invention, luminescent nucleotides 235, 245 or nanoparticles 315 may be detected using photodetectors 257 or electrical detectors 310. Apparatus 100 and sub-devices 200, 300 of use for nucleic acid 230, 310 sequencing 150 and/or identification 160 are also disclosed herein.
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Citations
48 Claims
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1. A method comprising:
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a) placing a template nucleic acid into four chambers, each chamber being associated with different nanopores and each chamber to comprising a different of labeled nucleotide;
b) synthesizing a labeled nucleic acid from the template nucleic acid;
c) contacting the labeled nucleic acid in each chamber with a its associated nanopore, wherein the labeled nucleic acid passes through the associated nanopore;
d) detecting a labeled nucleotide;
e) compiling a nucleotide distance map for each type of labeled nucleotide; and
f) determining the sequence of the nucleic acid from the nucleotide distance maps. - View Dependent Claims (2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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3. (canceled)
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14-30. -30. (canceled)
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31. A method comprising:
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a) placing a template nucleic acid into four sub-devices, each sub-device comprising an upper chamber and a lower chamber separated by a sensor layer having one or more nanopores, the upper and lower chambers of each sub-device in fluid communication through the nanopores, and each upper chamber containing a different labeled nucleotide;
b) synthesizing a labeled nucleic acid from the template nucleic acid in each upper chamber;
c) passing the labeled nucleic acid in each upper chamber through its associated nanopore;
d) detecting labeled nucleotides passing through the associated nanopore with a detector, the detector being operably coupled to the associated nanopore; and
e) compiling a nucleotide distance map for each type of labeled nucleotide. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48)
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Specification