Detection card for analyzing a sample for a target nucleic acid molecule, and uses thereof
First Claim
1. A detection card comprising:
- a first planar element comprising a detection chip, wherein the detection chip comprises;
two or more electrically separated conductors fabricated on a substrate, and capture probes attached to the conductors such that a gap exists between the capture probes on the conductors, wherein a sample, potentially containing a target molecule, can be analyzed for the presence of the target molecule by determining whether the conductors are electrically connected; and
a second planar element parallel to and joined with the first planar element along a first planar interface, wherein a fluid pathway is formed proximate to the first planar interface, said fluid pathway including a detection reservoir into which at least part of the detection chip is received.
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to a detection card made of a first planar element having a detection chip. The detection chip has two or more electrically separated conductors fabricated on a substrate and capture probes attached to the conductors, such that a gap exists between the capture probes on the conductors. A sample, potentially containing a target molecule, can be analyzed for the presence of the target molecule by determining whether the conductors are electrically connected. The detection card also has a second planar element parallel to and joined with the first planar element along a first planar interface. A fluid pathway is formed proximate to the first planar interface and includes a detection reservoir into which at least part of the detection chip is received. Also disclosed is a system and a method for detecting a target molecule, both of which utilize the detection card.
-
Citations
34 Claims
-
1. A detection card comprising:
-
a first planar element comprising a detection chip, wherein the detection chip comprises;
two or more electrically separated conductors fabricated on a substrate, and capture probes attached to the conductors such that a gap exists between the capture probes on the conductors, wherein a sample, potentially containing a target molecule, can be analyzed for the presence of the target molecule by determining whether the conductors are electrically connected; and
a second planar element parallel to and joined with the first planar element along a first planar interface, wherein a fluid pathway is formed proximate to the first planar interface, said fluid pathway including a detection reservoir into which at least part of the detection chip is received. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
-
-
22. A system for detecting a target molecule in a sample, said system comprising:
-
a detection card comprising;
a first planar element comprising a detection chip, wherein the detection chip comprises;
two or more electrically separated conductors fabricated on a substrate, and capture probes attached to the conductors such that a gap exists between the capture probes on the conductors, wherein a sample, potentially containing a target molecule, can be analyzed for the presence of the target molecule by determining whether the conductors are electrically connected; and
a second planar element parallel to and joined with the first planar element along a first planar interface, wherein a fluid pathway is formed proximate to the first planar interface, said fluid pathway including a detection reservoir into which at least part of the detection chip is received;
a first injection port through which a sample can be introduced into the detection card;
electrical connectors coupled to the electrically separated conductors of the detection chip, such that the presence of a target molecule connecting the capture probes on the electrically separated conductors can be detected; and
a support unit with respect to which the detection card can be positioned to carry out a procedure for detecting the target molecule in a sample, wherein said support unit has an electrical coupler suitable for electrical communication with the electrical connectors of the detection card, whereby the presence of the target molecule in the sample can be detected by the detection card and the support unit collectively. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29)
-
-
30. A method of detecting a target molecule comprising:
-
providing a detection system comprising;
a detection card comprising;
a first planar element comprising a detection chip, wherein the detection chip comprises;
two or more electrically separated conductors fabricated on a substrate, and capture probes attached to the conductors such that a gap exists between the capture probes on the conductors, wherein a sample, potentially containing a target molecule, can be analyzed for the presence of the target molecule by determining whether the conductors are electrically connected; and
a second planar element parallel to and joined with the first planar element along a first planar interface, wherein a fluid pathway is formed proximate to the first planar interface, said fluid pathway including a detection reservoir into which at least part of the detection chip is received;
a first injection port through which a sample can be introduced into the detection reservoir;
electrical connectors coupled to the electrically separated conductors of the detection chip, such that the presence of a target molecule connecting the capture probes on the electrically separated conductors can be detected; and
a support unit with respect to which the detection card can be positioned to carry out a procedure for detecting the target molecule in a sample, wherein said support unit has an electrical coupler suitable for electrical communication with the electrical connector of the detection card;
injecting a sample, potentially containing the target molecule, into the first injection port;
processing the sample within the detection reservoir under conditions effective to permit any of the target molecule present in the sample to bind to the capture probes and thereby connect the capture probes; and
detecting the presence of the target molecule by determining whether electricity is conducted between the electrically separated conductors. - View Dependent Claims (31, 32, 33, 34)
-
Specification