Detection card for analyzing a sample for a target nucleic acid molecule, and uses thereof

• US 20060019273A1
• Filed: 05/12/2005
• Published: 01/26/2006
• Est. Priority Date: 05/12/2004
• Status: Abandoned Application

• Current Assignee: Craig W. Tiller, Dennis M. Connolly, Jeffrey Hainon, Richard S. Murante, Roberta J. Greco
• Original Assignee: Craig W. Tiller, Dennis M. Connolly, Jeffrey Hainon, Richard S. Murante, Roberta J. Greco
• Inventors: Connolly, Dennis M., Murante, Richard S., Greco, Roberta J., Tiller, Craig W., Hainon, Jeffrey
• Application Number:
  US11/128,079
• Publication Number:
  US 20060019273A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0605 check valves
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip

Abstract

The present invention relates to a detection card made of a first planar element having a detection chip. The detection chip has two or more electrically separated conductors fabricated on a substrate and capture probes attached to the conductors, such that a gap exists between the capture probes on the conductors. A sample, potentially containing a target molecule, can be analyzed for the presence of the target molecule by determining whether the conductors are electrically connected. The detection card also has a second planar element parallel to and joined with the first planar element along a first planar interface. A fluid pathway is formed proximate to the first planar interface and includes a detection reservoir into which at least part of the detection chip is received. Also disclosed is a system and a method for detecting a target molecule, both of which utilize the detection card.

Claims

1. A detection card comprising:

a first planar element comprising a detection chip, wherein the detection chip comprises;

two or more electrically separated conductors fabricated on a substrate, and

capture probes attached to the conductors such that a gap exists between the capture probes on the conductors, wherein a sample, potentially containing a target molecule, can be analyzed for the presence of the target molecule by determining whether the conductors are electrically connected; and

a second planar element parallel to and joined with the first planar element along a first planar interface, wherein a fluid pathway is formed proximate to the first planar interface, said fluid pathway including a detection reservoir into which at least part of the detection chip is received.

2. The detection card according to claim 1, wherein the two or more electrically separated conductors are in a form of spaced, apart conductive fingers.

3. The detection card according to claim 1, wherein a plurality of pairs of spaced apart conductive fingers are present on the detection chip.

4. The detection card according to claim 1, wherein the capture probes are oligonucleotides.

5. The detection card according to claim 1, wherein the capture probes are peptide nucleic acid analogs.

6. The detection card according to claim 1, wherein the fluid pathway further comprises:

a first injection port through which a sample can be introduced into the detection card.

7. The detection card according to claim 6, wherein the fluid pathway further comprises:

a first channel which connects the first injection port to the detection reservoir.

8. The detection card according to claim 7, wherein the fluid pathway further comprises:

a one-way valve in the first channel, permitting fluid only to flow from the first injection port to the detection reservoir.

9. The detection card according to claim 7, wherein the fluid pathway further comprises:

an archival reservoir positioned in the first channel between the first injection port and the detection reservoir.

10. The detection card according to claim 9 further comprising:

at least two one-way valves in the first channel upstream and downstream of the archival reservoir, permitting fluid only to flow from the first injection port to the detection reservoir.

11. The detection card according to claim 6, wherein the fluid pathway further comprises:

a waste reservoir for collecting material discharged from the detection reservoir and

a second channel establishing fluid communication between the detection reservoir and the waste reservoir.

12. The detection card according to claim 6, wherein the detection chip further comprises:

a heating element.

13. The detection card according to claim 6 further comprising:

a DNA concentrator upstream of the detection reservoir.

14. The detection card according to claim 6 further comprising:

electrical connectors extending through the detection card and coupled to the electrically separated conductors of the detection chip.

15. The detection card according to claim 6, wherein the fluid pathway further comprises:

a plurality of injection ports through which reagents can be introduced into the detection card.

16. The detection card according to claim 1, wherein the fluid pathway further comprises:

a plurality of reservoirs suitable for holding reagents and positioned to discharge the reagents into the detection reservoir.

17. The detection card according to claim 16, wherein the reservoirs comprise:

a reservoir carrying a neutralizer;

a reservoir carrying a buffer;

a reservoir carrying a conductive ion solution; and

a reservoir carrying an enhancer.

18. The detection card according to claim 16, wherein each of the plurality of reservoirs is connected to the detection reservoir by a plurality of channels.

19. The detection card according to claim 18 further comprising:

a one-way valve in each of the plurality of channels, permitting fluid only to flow from each of the plurality of reservoirs to the detection reservoir.

20. The detection card according to claim 1, wherein the second planar element has an indented pattern so that the fluid pathway is formed by the indented pattern in the second planar element and a surface of the first planar element which forms the first planar interface.

21. The detection card according to claim 1 further comprising:

a third planar element, wherein the second planar element has a cut-out pattern and is positioned between the first planar element and the third planar element with the second and third planar elements being joined together along a second planar interface, the fluid pathway being formed by the cut-out pattern in the second planar element and surfaces of the first and third planar elements which form the first and second planar interfaces respectively.

22. A system for detecting a target molecule in a sample, said system comprising:

a detection card comprising;

a first planar element comprising a detection chip, wherein the detection chip comprises;

two or more electrically separated conductors fabricated on a substrate, and

capture probes attached to the conductors such that a gap exists between the capture probes on the conductors, wherein a sample, potentially containing a target molecule, can be analyzed for the presence of the target molecule by determining whether the conductors are electrically connected; and

a second planar element parallel to and joined with the first planar element along a first planar interface, wherein a fluid pathway is formed proximate to the first planar interface, said fluid pathway including a detection reservoir into which at least part of the detection chip is received;

a first injection port through which a sample can be introduced into the detection card;

electrical connectors coupled to the electrically separated conductors of the detection chip, such that the presence of a target molecule connecting the capture probes on the electrically separated conductors can be detected; and

a support unit with respect to which the detection card can be positioned to carry out a procedure for detecting the target molecule in a sample, wherein said support unit has an electrical coupler suitable for electrical communication with the electrical connectors of the detection card, whereby the presence of the target molecule in the sample can be detected by the detection card and the support unit collectively.

23. The system according to claim 22, wherein the support unit has a slot into which the detection card is inserted.

24. The system according to claim 22, wherein the support unit is portable.

25. The system according to claim 24, wherein the support unit is a personal digital assistant.

26. The system according to claim 22, wherein the support unit is a desk-top unit.

27. The system according to claim 26, wherein the support unit further comprises:

a pump to remove reagents from the plurality of reservoirs and feed them into the detection reservoir through the plurality of injection ports.

28. The system according to claim 26, wherein the support unit further comprises:

a controller in communication with the electrical connectors to enable electrical communication between the electrical conductors of the detection card and the controller, whereby the presence of the target molecule in the sample can be detected by the detection card and support unit collectively.

29. The system according to claim 28, wherein the support unit further comprises:

a digital coupling to permit the controller to communicate data to a computer external of the support unit.

30. A method of detecting a target molecule comprising:

providing a detection system comprising;

a detection card comprising;

a first planar element comprising a detection chip, wherein the detection chip comprises;

two or more electrically separated conductors fabricated on a substrate, and

capture probes attached to the conductors such that a gap exists between the capture probes on the conductors, wherein a sample, potentially containing a target molecule, can be analyzed for the presence of the target molecule by determining whether the conductors are electrically connected; and

a second planar element parallel to and joined with the first planar element along a first planar interface, wherein a fluid pathway is formed proximate to the first planar interface, said fluid pathway including a detection reservoir into which at least part of the detection chip is received;

a first injection port through which a sample can be introduced into the detection reservoir;

electrical connectors coupled to the electrically separated conductors of the detection chip, such that the presence of a target molecule connecting the capture probes on the electrically separated conductors can be detected; and

a support unit with respect to which the detection card can be positioned to carry out a procedure for detecting the target molecule in a sample, wherein said support unit has an electrical coupler suitable for electrical communication with the electrical connector of the detection card;

injecting a sample, potentially containing the target molecule, into the first injection port;

processing the sample within the detection reservoir under conditions effective to permit any of the target molecule present in the sample to bind to the capture probes and thereby connect the capture probes; and

detecting the presence of the target molecule by determining whether electricity is conducted between the electrically separated conductors.

31. The method according to claim 30 further comprising:

collecting material discharged from the detection reservoir in a waste reservoir.

32. The method according to claim 30, wherein said processing comprises:

neutralizing the sample; and

.

contacting the neutralized sample with a buffer, said method further comprising;

treating the sample with a conductive ion solution after said contacting with a buffer and

treating the sample with an enhancer after said treating with a conductive ion solution.

33. The method according to claim 30 further comprising:

coating any target molecules bound to the capture probes with a conductive ion material after said processing and before said detecting.

34. The method according to claim 30 further comprising:

inserting the detection card into a slot within the support unit.