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Methods for nucleic acid amplification and sequence determination

  • US 20060024711A1
  • Filed: 06/23/2005
  • Published: 02/02/2006
  • Est. Priority Date: 07/02/2004
  • Status: Abandoned Application
First Claim
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1. A method of determining a sequence of a nucleic acid, the method comprising the steps of:

  • (a) conducting rolling circle amplification of a nucleic acid to produce an amplicon comprising about two to about one hundred linked complements of a nucleic acid;

    wherein said amplicon is anchored to a substrate, such that said amplicon is individually optically resolvable; and

    (b) determining a sequence of at least a portion of said nucleic acid.

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