×

Assay and kit for analyzing gene expression

  • US 20060024721A1
  • Filed: 08/01/2005
  • Published: 02/02/2006
  • Est. Priority Date: 01/24/2001
  • Status: Abandoned Application
First Claim
Patent Images

1. A kit for performing or assaying expression profiling of polynucleotides by determining the presence or sequence of a single stranded polynucleotide tag, said kit comprising a) at least one site-specific nicking endonuclease capable of i) recognizing a predetermined nucleotide motif comprising complementary nucleotide strands and ii) cleaving only one of said complementary strands, said cleavage generating at least one single stranded polynucleotide tag, b) at least one adapter oligonucleotide comprising a recognition motif for said site-specific nicking endonuclease, and c) at least one identifying linker oligonucleotide for identifying said at least one single stranded polynucleotide tag.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×