Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
First Claim
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1. A method for identifying a target nucleotide sequence comprising:
- forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture;
amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture;
detecting the amplified ligation product; and
identifying the target nucleotide sequence.
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Abstract
The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
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Citations
9 Claims
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1. A method for identifying a target nucleotide sequence comprising:
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forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture;
amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture;
detecting the amplified ligation product; and
identifying the target nucleotide sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification