Normalization of data using controls
First Claim
1. A calibration apparatus for calibrating an instrument, the instrument capable of polymerase chain reaction, the apparatus comprising:
- a microplate comprising at least 400 wells; and
a plurality of dyes at known concentrations in at least some of the at least 400 wells.
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Accused Products
Abstract
An apparatus for normalizing a PCR instrument, can comprise a microplate comprising at least 6,000 wells and a system of dyes at known concentrations in a plurality of the at least 6,000 wells. A method for normalizing a system can comprise (a) providing a microplate comprising at least 6,000 wells and a system of dyes; (b) exciting at least one dye; (c) detecting an emission output for the at least one dye; (d) determining if the emission output is in an acceptable range; and (e) adjusting the system so that the emission output is in the acceptable range.
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Citations
22 Claims
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1. A calibration apparatus for calibrating an instrument, the instrument capable of polymerase chain reaction, the apparatus comprising:
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a microplate comprising at least 400 wells; and
a plurality of dyes at known concentrations in at least some of the at least 400 wells. - View Dependent Claims (2, 3, 4, 5)
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6. A method for calibrating an instrument, the method comprising:
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providing a first instrument;
placing a microplate comprising at least 6,000 wells and a plurality of dyes in the first instrument;
exciting at least one dye of the plurality of dyes;
detecting an emission output from the at least one dye;
determining if the emission output from the at least one dye is within a predetermined range; and
calibrating the first instrument so that the emission output from the at least one dye is within the predetermined range. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for normalizing a first PCR system as compared to a second PCR system, the method comprising:
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providing a first PCR system;
placing a microplate comprising at least 400 wells and a plurality of dyes in at least one of the at least 400 wells into the first PCR system;
exciting at least one dye of the plurality of dyes;
detecting an emission output from the at least one dye;
evaluating an adjustment to the first PCR system based on the emission output for the at least one dye so that the first PCR system is normalized to the second PCR system; and
adjusting the first PCR system operably normalizing it to the second PCR system. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
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Specification