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Methods for improving cell line activity in immunoisolation devices

  • US 20060029584A1
  • Filed: 09/27/2005
  • Published: 02/09/2006
  • Est. Priority Date: 06/08/2001
  • Status: Abandoned Application
First Claim
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1. A method for optimizing cell survival in an immunoisolation device implantation in a recipient animal, said method comprising the steps of:

  • (a) loading cells into a first immunoisolation device;

    (b) implanting said first immunoisolation device into a host animal;

    (c) removing said first immunoisolation device from said host animal after a period of time;

    (d) unloading the cells from said removed first immunoisolation device;

    (e) expanding said unloaded cells on medium supporting growth of said cells;

    (f) loading said expanded cells into a second immunoisolation device;

    (g) optionally repeating steps (b)-(f) for one or more times;

    wherein the loaded second immunoisolation device of step (f) contains cell lines optimized for survival in an implantation in said recipient animal.

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