Methods for improving cell line activity in immunoisolation devices
First Claim
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1. A method for optimizing cell survival in an immunoisolation device implantation in a recipient animal, said method comprising the steps of:
- (a) loading cells into a first immunoisolation device;
(b) implanting said first immunoisolation device into a host animal;
(c) removing said first immunoisolation device from said host animal after a period of time;
(d) unloading the cells from said removed first immunoisolation device;
(e) expanding said unloaded cells on medium supporting growth of said cells;
(f) loading said expanded cells into a second immunoisolation device;
(g) optionally repeating steps (b)-(f) for one or more times;
wherein the loaded second immunoisolation device of step (f) contains cell lines optimized for survival in an implantation in said recipient animal.
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Abstract
Methods for maintaining and improving the secretory activity of cells housed in immunoisolation devices.
8 Citations
51 Claims
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1. A method for optimizing cell survival in an immunoisolation device implantation in a recipient animal, said method comprising the steps of:
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(a) loading cells into a first immunoisolation device;
(b) implanting said first immunoisolation device into a host animal;
(c) removing said first immunoisolation device from said host animal after a period of time;
(d) unloading the cells from said removed first immunoisolation device;
(e) expanding said unloaded cells on medium supporting growth of said cells;
(f) loading said expanded cells into a second immunoisolation device;
(g) optionally repeating steps (b)-(f) for one or more times;
wherein the loaded second immunoisolation device of step (f) contains cell lines optimized for survival in an implantation in said recipient animal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for selecting cells with optimal desired functionality in an immunoisolation device implantation in a recipient animal, said method comprising the steps of:
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(a) loading cells having the desired functionality into a plurality of first immunoisolation devices;
(b) implanting said first immunoisolation devices into a plurality of host animals;
(c) monitoring said host animals for said cellular functionality;
(d) removing said immunoisolation devices from said host animals suggesting a predetermined level of cellular functionality;
(e) unloading the cells from said removed immunoisolation devices onto a plurality of medium supports supporting growth of said unloaded cells;
(f) expanding said unloaded cells on said medium supports;
(g) determining said medium supports that contain cells having a predetermined level of desired cellular functionality;
(h) loading said expanded cells having said predetermined level of desired cellular functionality into one or more second immunoisolation device(s); and
(i) optionally repeating steps (b)-(h) for one or more times;
wherein the loaded immunoisolation device of step (h) contains cell lines having optimal desired functionality for immunoisolation device implantation into said recipient animal. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 51)
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30. A method for optimizing cell survival in an immunoisolation device implantation in a recipient animal, said method comprising the steps of:
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(a) loading cells into a first immunoisolation device;
(b) culturing said first immunoisolation device into a culture vessel;
(c) removing said first immunoisolation device from said culture vessel after a period of time;
(d) unloading the cells from said removed first immunoisolation device;
(e) expanding said unloaded cells on medium supporting growth of said cells;
(f) loading said expanded cells into a second immunoisolation device; and
(g) optionally repeating steps (b)-(f) for one or more times;
wherein the loaded second immunoisolation device of step (f) contains cell lines optimized for survival in an immunoisolation device cultured in a culture vessel. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 38)
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39. A method for selecting cells with optimal desired functionality in an immunoisolation device implantation in a recipient animal, said method comprising the steps of:
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(a) loading cells having the desired functionality into a plurality of first vascularizing immunoisolation devices;
(b) culturing said first immunoisolation devices into a plurality of culture vessels;
(c) monitoring culture medium said culture vessels for said cellular functionality;
(d) removing said immunoisolation devices from said culture vessels suggesting a predetermined level of cellular functionality;
(e) unloading the cells from said removed immunoisolation device onto a plurality of medium supports supporting growth of said unloaded cells;
(f) expanding said unloaded cells on said medium supports;
(g) determining said medium supports that contain cells having a predetermined level of desired cellular functionality;
(h) loading said expanded cells having said predetermined level of desired cellular functionality into one or more second vascularizing immunoisolation device(s); and
(i) optionally repeating steps (b)-(h) for one or more times;
wherein the loaded immunoisolation device of step (h) contains cell lines having optimal desired functionality for immunoisolation device implantation into said recipient animal. - View Dependent Claims (40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
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Specification