System for fluorescence monitoring
First Claim
1. A system for performing PCR and monitoring the reaction during temperature cycling comprising:
- a sample container for holding a PCR sample, a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the PCR sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector configured for detecting fluorescent emissions from SYBR™
Green I, and a processor for recording and processing emissions from SYBR®
Green I.
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Abstract
The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
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Citations
22 Claims
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1. A system for performing PCR and monitoring the reaction during temperature cycling comprising:
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a sample container for holding a PCR sample, a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the PCR sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector configured for detecting fluorescent emissions from SYBR™
Green I, anda processor for recording and processing emissions from SYBR®
Green I. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A device for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye comprising:
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a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to generate a melting curve during or subsequent to amplification. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification