Test plate and test method using the same

• US 20060034727A1
• Filed: 07/15/2005
• Published: 02/16/2006
• Est. Priority Date: 08/13/2004
• Status: Abandoned Application

• Current Assignee: ALPS Electric Company Limited (Alps Alpine Co., Ltd.)
• Original Assignee: ALPS Electric Company Limited (Alps Alpine Co., Ltd.)
• Inventors: Shibuya, Yoshihisa, Yamashita, Tatsumaro, Takamura, Shozo
• Application Number:
  US11/183,088
• Publication Number:
  US 20060034727A1
• Time in Patent Office: Days
• US Class Current: 422/58
• CPC Class Codes: B01F 33/3021 the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
• CPC Class Codes: B01F 35/71 Feed mechanisms with proportioning B01F35/80
• CPC Class Codes: B01F 35/712 for feeding fluids
• CPC Class Codes: B01F 35/7172 using capillary forces
• CPC Class Codes: B01F 35/71745 using pneumatic pressure, overpressure, gas or air pressure in a closed receptacle or circuit system
• CPC Class Codes: B01F 35/7543 using pneumatic pressure, overpressure or gas pressure in a closed receptacle or circuit system
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0442 thermal energy, e.g. vaporisation, bubble jet
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043

Abstract

A test plate includes a plate substrate and a lid body. The plate substrate includes a flow path; an upstream storage chamber that is connected to the upstream side of the flow path and stores an upstream material; and a downstream storage chamber that is connected to the downstream side of the flow path and stores a downstream material. At least a portion of a surface of a space from the upstream storage chamber to the downstream storage chamber through the flow path is composed of a water-repellent surface.

Claims

1. A test plate comprising:

a plate substrate; and

a lid body,

wherein the plate substrate includes;

a flow path;

an upstream storage chamber that is connected to the upstream side of the flow path and stores an upstream material; and

a downstream storage chamber that is connected to the downstream side of the flow path and stores a downstream material, and

wherein at least a portion of a surface constituting a space from the upstream storage chamber to the downstream storage chamber through the flow path is composed of a water-repellent surface.

2. The test plate according to claim 1,

wherein the water-repellent surface is formed on the entire surface constituting the space from the upstream storage chamber to the downstream storage chamber through the flow path.

3. The test plate according to claim 1,

wherein the water-repellent surface is formed by coating the surface constituting the space with a water-repellent agent.

4. The test plate according to claim 1,

wherein the plate substrate and/or the lid body contains a water-repellent agent, so that the surface is composed of a water-repellent surface.

5. The test plate according to claim 1,

wherein the upstream storage chamber is connected to a pressure transmission member, and the downstream storage chamber is connected to a path for releasing the pressure from the pressure transmission member to the outside.

6. The test plate according to claim 5,

wherein the diameter of the path is smaller than the diameter of the flow path.

7. A test method using a test plate,

the test plate including:

a plate substrate; and

a lid body,

wherein the plate substrate includes;

a flow path;

an upstream storage chamber that is connected to the upstream side of the flow path and stores an upstream material; and

a downstream storage chamber that is connected to the downstream side of the flow path and stores a downstream material, and

wherein at least a portion of a surface constituting a space from the upstream storage chamber to the downstream storage chamber through the flow path is composed of a water-repellent surface,

the test method comprising;

previously storing the upstream material in the upstream storage chamber of the test plate so that at least the upstream material is repelled by the water-repellent surface and is maintained so as not to reach the downstream storage chamber;

storing the downstream material in the downstream storage chamber; and

sending the upstream material to the downstream storage chamber by using a predetermined-means so that the upstream material and the downstream material are mixed with each other in the downstream storage chamber.

8. The test method using a test plate according to claim 7,

wherein, after the downstream material is stored, the upstream material is then sent to the downstream storage chamber by using the pressure transmission member.

9. The test method using a test plate according to claim 7,

wherein the upstream material is a reagent, and the downstream material is a test sample.

10. The test method using a test plate according to claim 7,

wherein the upstream material is beads on which probes are fixed.

11. The test method using a test plate according to the claim 10,

wherein the diameter of the bead is larger than the diameter of the path connected to the downstream storage chamber, and the bead is stemmed inside the downstream storage chamber.