Method and apparatus for molecular analysis in small sample volumes
First Claim
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1. An apparatus for analyzing a sample comprising a probe having a plurality of domains disposed thereon, wherein the domains form an array.
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Abstract
The interrogation of extremely small sample volumes can be accomplished with the present invention. Provided are probes having disposed thereon a plurality of domains forming an array, which is suitably a nanoarray. Also provided are methods of detecting molecules and molecular interaction events, retrieving and analyzing analytes, and delivering substances to cells or tissues using probes of the invention.
104 Citations
27 Claims
- 1. An apparatus for analyzing a sample comprising a probe having a plurality of domains disposed thereon, wherein the domains form an array.
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14. A method of detecting a molecular interaction event comprising:
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contacting a sample with a probe having a plurality of domains disposed in an array;
providing an incubation period;
washing unbound molecules from the domains; and
detecting the molecular interaction event. - View Dependent Claims (15, 16)
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17. A method of detecting one or more molecules in a sample comprising:
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contacting the sample a probe having a plurality of domains disposed thereon, wherein the domains form an array, and wherein the domains are operably connected to one or more nanosensors; and
detecting binding of one or more molecules to one or more of the domains.
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18. A method of analyzing one or more analytes in a cell comprising:
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disrupting a cell with a microdisrupter disposed on a probe, wherein the probe has a plurality of domains disposed thereon, and wherein the domains form a nanoarray;
passing the nanoarray through the membrane of the cell such that the nanoarray contacts intracellular space; and
detecting the binding of one or more analytes to the nanoarray. - View Dependent Claims (19, 20, 21)
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22. A method of retrieving at least one analyte from a sample comprising:
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contacting the sample with a probe having a plurality of domains disposed thereon, wherein the domains form an array; and
retrieving at least one analyte from the molecular domains.
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23. A method of delivering at least one substance to a cell comprising:
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reversibly attaching at least one substance to a probe having a plurality of domains disposed thereon, wherein the domains form an array;
passing the probe through the membrane of the cell into the intracellular space; and
releasing at least one substance into the intracellular space. - View Dependent Claims (24, 25, 26)
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27. A method of detecting an in situ molecular interaction event comprising:
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contacting a sample with a probe having a plurality of domains disposed in an array;
providing an incubation period; and
detecting the molecular interaction event.
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Specification