Assays employing electrochemiluminescent labels and electrochemiluminescence quenchers
First Claim
1. A method for detecting an analyte in a sample composition comprising the steps of:
- (a) preparing an assay mixture comprising;
said sample composition;
a reagent having an ECL label; and
, a reagent having an ECL quenching moiety, said ECL quenching moiety comprising at least one benzene moiety, (b) determining any difference between the ECL emissions of;
(i) the assay mixture prepared in step (a); and
, (ii) an assay mixture comprising;
said reagent having an ECL label;
said reagent having an ECL quenching moiety; and
, a known amountoisaid analyte; and
, (c) correlating any difference determined in step (b) with the amount of analyte in said sample.
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Abstract
This invention pertains to the general field of chemical and biological assays which employ electrochemiluminescence (ECL), also referred to as electrogenerated chemiliminescence. More particularly, the present invention pertains to certain classes of chemical moieties which strongly quench ECL, and the use of these ECL quenchers in combination with ECL labels, for example, in ECL assay methods which employ an ECL quencher and an ECL label. One class of such quenching moieties are those which comprise at least one benzene moiety. Sub-classes of such quenching moieties are those which comprise at least one phenol moiety, quinone moiety, benzene carboxylic acid, and/or benzene carboxylate moiety.
22 Citations
28 Claims
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1. A method for detecting an analyte in a sample composition comprising the steps of:
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(a) preparing an assay mixture comprising;
said sample composition;
a reagent having an ECL label; and
,a reagent having an ECL quenching moiety, said ECL quenching moiety comprising at least one benzene moiety, (b) determining any difference between the ECL emissions of;
(i) the assay mixture prepared in step (a); and
,(ii) an assay mixture comprising;
said reagent having an ECL label;
said reagent having an ECL quenching moiety; and
,a known amountoisaid analyte; and
,(c) correlating any difference determined in step (b) with the amount of analyte in said sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 25, 26, 27, 28)
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Specification