Methods for correcting mitotic spindle defects and optimizing preimplantation embryonic developmental rates associated with somatic cell nuclear transfer in animals
First Claim
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1. A method comprising the steps of:
- introducing nuclei along with one or more molecular components into an extrusion-enucleated egg, thus creating a nuclear transfer construct;
culturing said nuclear transfer construct to produce a viable embryo;
transferring said embryo to the oviducts of a female; and
producing a cloned animal.
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Abstract
The present invention is directed to various methodologies to make NT a practical procedure for animals, specifically, primates including human and non-human primates. Furthermore, the methods and molecular components provided by the present invention provide a practical means for producing embryos with desired characteristics. In a specific embodiment, the methodology of the present invention comprises introducing nuclei having desired characteristics along with one or more molecular components into an enucleated egg, thus creating a nuclear transfer construct, culturing the egg to produce a viable embryo, transferring the embryo to the oviducts of a female, and producing a cloned animal.
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Citations
56 Claims
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1. A method comprising the steps of:
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introducing nuclei along with one or more molecular components into an extrusion-enucleated egg, thus creating a nuclear transfer construct;
culturing said nuclear transfer construct to produce a viable embryo;
transferring said embryo to the oviducts of a female; and
producing a cloned animal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55)
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56-214. -214. (canceled)
Specification