Low volume filtration column devices and methods of filtering therewith

• US 20060037903A1
• Filed: 03/09/2005
• Published: 02/23/2006
• Est. Priority Date: 06/15/2001
• Status: Active Grant

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: MDS Analytical Technologies US Incorporated (Danaher Corporation)
• Inventors: Richardson, Derrick, Smith, Thomas L., Monteon, Jorge, Kunitake, Steven T., Pai, Derek S.
• Granted Patent: US 7,556,733 B2
• Time in Patent Office: Days
• US Class Current: 210/240
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 2300/043 Hinged closures
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/048 enabling gas exchange, e.g. vents
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: B01L 3/5082 Test tubes per se
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 1/405 by adsorption or absorption

Abstract

This relates to filter columns for isolating nucleic acids, particularly at small elution volumes. The filter column is adapted for stable placement within the upper portion of standard plastic collection tubes of various sizes. The body of the filter column has a number of surfaces to accommodate placement within variously sized collection tubes. The filter column contains nucleic acid-specific filter which can be located at alternate regions within the filter column, providing different filter surface areas and loading volume capacities using the same column body. The filter column has an opening on an upper end adapted to be sealed by a cap. A method for recovering nucleic acids using such filter column is also provided.

Claims

1. A filter column, comprising:

at least one body portion having a passageway extending through the at least one body portion;

the body having a first end, a second end, an outer surface and an inner surface;

a filter located in the passageway;

a lid for closing one end of the passageway; and

at least one vent located in the lid.

2. The filter column of claim 1 wherein the at least one vent includes a curved surface.

3. The filter column of claim 1 wherein the at least one vent is tapered.

4. The filter column of claim 3 wherein the at least one vent is tapered such that the vent is narrower towards the top of the lid.

5. The filter column of claim 1 wherein the at least one vent includes a curved surface and is tapered.

6. The filter column of claim 5 wherein the at least one vent is tapered such that the vent is narrower towards the top of the lid.

7. The filter column of claim 1 wherein the at least one vent prevents fluid from being pushed through the filter when the lid is closed down onto the column.

8. The filter column of claim 1 wherein the at least one vent prevents fluid from escaping from around or through the lid.

9. The filter column of claim 1 wherein the at least one vent prevents fluid from being pushed through the filter when the lid is closed down onto the column and prevents fluid from escaping from around or through the lid.

10. The filter column of claim 1 further including at least one bearing surface on an outer surface for seating on at least one collection tube.

11. The filter column of claim 10 where the outer surface further includes a second bearing surface for seating on at least a second collection tube that has a different volume from the at least one collection tube.

12. The filter column of claim 1 further including at least a first body portion and a second body portion;

wherein the cross-sectional area of the passageway at the first body portion is greater than the cross-sectional area of the passageway at the second body portion.

13. The filter column of claim 12 wherein the filter is located in the second body portion.

14. The filter column of claim 1 wherein the lid is connected to the body with a hinge.

15. A filter column, comprising:

a body having a passageway extending therethrough, the body having a first end, a second end and a longitudinal axis, an outer surface and an inner surface;

the inner surface having at least one angled surface;

the at least one angled surface forming an angle with the longitudinal axis that is approximately 30 degrees; and

a filter disposed within the passageway.

16. The filter column of claim 15 further including at least one bearing surface on an outer surface for seating on at least one collection tube.

17. The filter column of claim 15 further including a second bearing surface for seating on at least a second collection tube that has a different volume from the at least one collection tube.

18. The filter column of claim 15 further including at least a first body portion and a second body portion;

wherein the cross-sectional area of the passageway at the first body portion is greater than the cross-sectional area of the passageway at the second body portion.

19. The filter column of claim 18 wherein the at least one angled surface is located between the first body portion and the second body portion.

20. The filter column of claim 18 the filter column of claim 18 wherein the filter is located in the second body portion.