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Microarrays utilizing hydrogels

  • US 20060040274A1
  • Filed: 08/19/2004
  • Published: 02/23/2006
  • Est. Priority Date: 08/19/2004
  • Status: Active Grant
First Claim
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1. A method of making a microarray, which method comprises the steps of:

  • providing a substrate having an upper surface that is functionalized with organic molecules, coating said surface with a polymerizable hydrogel layer so as to completely cover an array region of the surface, said layer including anchoring moieties dispersed uniformly therethroughout, curing said coated substrate to polymerize said hydrogel layer, and subsequently attaching a plurality of different probes to said coated surface at distinct spatial locations thereon by linking said probes to said anchoring moieties which are present within said cured hydrogel layer.

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