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Test device and method for colored particle immunoassay

  • US 20060040405A1
  • Filed: 01/12/2005
  • Published: 02/23/2006
  • Est. Priority Date: 06/27/1988
  • Status: Abandoned Application
First Claim
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1. A method of detecting a ligand in a liquid sample, the method comprising the steps of:

  • A. transporting along a flow path in a test cell a solution, including a liquid sample suspected to contain a ligand and a conjugate, into contact with a test site visible through a window in a wall of said test cell, said test site having immobilized thereon a first protein having a binding site specific to a first epitope on the ligand, said conjugate comprising colored particles coupled to a second protein selected from the group consisting of proteins having a binding site specific to a second epitope on the ligand and proteins which bind with said first protein in competition with the ligand, and B. continuing transport of said solution to progressively produce at said test site a complex comprising said ligand for a time sufficient to visually determine through said window whether a color is developed at said test site;

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