Method of producing a plurality of isolated antibodies to a plurality of cognate antigens

• US 20060051348A1
• Filed: 09/07/2005
• Published: 03/09/2006
• Est. Priority Date: 09/09/2004
• Status: Abandoned Application

• Current Assignee: Montecito Bio Sciences, Ltd.
• Original Assignee: Montecito Bio Sciences, Ltd.
• Inventors: Gorlach, Jorn
• Application Number:
  US11/221,252
• Publication Number:
  US 20060051348A1
• Time in Patent Office: Days
• US Class Current: 424/133.100
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002

Abstract

The present invention relates to a method for producing high affinity antibodies that are antigen-specific. The method involves binding a plurality of antibody-producing B-cells from a mammal to a plurality of cognate antigens; sorting the bound antibody-producing B-cell and cognate antigen; amplifying nucleic acid sequences encoding each antibody, or fragment thereof, from the B-cells; and expressing the each antibody in a protein expression system. Antibodies produced in this manner are useful in diagnostic and therapeutic applications.

Claims

1. A method for producing a plurality of isolated antibodies to a plurality of cognate antigens comprising

binding a plurality of antibody-producing B-cells from a mammal to a plurality of cognate antigens;

isolating each bound antibody-producing B-cell and cognate antigen;

amplifying nucleic acid sequences encoding each antibody, or fragment thereof, from the B-cells;

introducing each nucleic acid sequence encoding each antibody, or fragment thereof, into an expression system capable of expressing an antibody so that a plurality of isolated antibodies to a plurality of cognate antigens is produced.

2. An antibody, or fragment thereof identified by the method of claim 1.