Device, method, and kit for gene detection
First Claim
1. A gene detection tool comprising a sealable reaction chamber and a sealable detection chamber and a partition member positioned between the reaction chamber and the detection chamber that separates the reaction chamber and the detection chamber, wherein the partition member is capable of unsealing;
- the reaction chamber is comprised of at least two members capable of forming a sealed reaction chamber by mutually fitting an opening means provided therein; and
the detection chamber or reaction chamber comprises a gene detection element.
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Accused Products
Abstract
A gene detection tool comprising a sealable reaction chamber and a sealable detection chamber and a partition member positioned between the reaction chamber and the detection chamber that separates the reaction chamber and the detection chamber. The partition member is capable of unsealing; the reaction chamber is comprised of at least two members capable of forming a sealed reaction chamber by mutually fitting an opening means provided therein; and the detection chamber or reaction chamber comprises a gene detection element. A gene detection method in which a gene amplification operation and a gene detection operation are conducted in a sealed state using a gene detection device comprising a sealable reaction chamber and a sealable detection chamber, having a partition member which is positioned between the reaction chamber and the detection chamber and which separates the reaction chamber from the detection chamber, with test sample and gene amplification reaction solution being packed in said reaction chamber and a gene-binding agent capable of binding with a gene and generating a detectable signal being packed in said detection chamber. (1) At least the reaction chamber is imparted with conditions capable of amplifying the gene to be detected; (2) following the operation of (1), the partition member is unsealed while maintaining the gene detection tool in a sealed state; (3) through a passage created in the partition member by the unsealing, the solution in the reaction chamber is brought into contact with the gene-binding agent capable of binding the gene in the detection chamber and generating a detectable signal; and (4) the signal obtained is detected. A gene detection kit comprising the gene detection tool, a gene amplification reaction solution or gene amplification reagent, and a gene-binding agent capable of binding a gene and generating a detectable signal.
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Citations
20 Claims
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1. A gene detection tool comprising a sealable reaction chamber and a sealable detection chamber and a partition member positioned between the reaction chamber and the detection chamber that separates the reaction chamber and the detection chamber, wherein
the partition member is capable of unsealing; -
the reaction chamber is comprised of at least two members capable of forming a sealed reaction chamber by mutually fitting an opening means provided therein; and
the detection chamber or reaction chamber comprises a gene detection element. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 19, 20)
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10. A gene detection tool comprising a mutually independent sealable reaction chamber and detection chamber, and a partition member positioned between the reaction chamber and the detection chamber that seals off the reaction chamber and detection chamber from each other, and is characterized in that:
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(a) the reaction chamber comprises an opening means permitting the insertion of a test sample potentially containing a gene to be detected and a gene amplification reaction solution;
(b) the detection chamber comprises an opening means permitting the insertion of a gene-binding agent that binds a gene and generates a detectable signal;
(c) the partition member is capable of unsealing by an operation from the exterior while maintaining the overall sealed state of the gene detection tool comprising the reaction chamber and detection chamber; and
(d) when the partition member is unsealed, the passage generated by this unsealing allows the solution in the reaction chamber to move into the detection chamber. - View Dependent Claims (11)
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12. A gene detection method in which a gene amplification operation and a gene detection operation are conducted in a sealed state using a gene detection device comprising a sealable reaction chamber and a sealable detection chamber, having a partition member which is positioned between the reaction chamber and the detection chamber and which separates the reaction chamber from the detection chamber, with test sample and gene amplification reaction solution being packed in said reaction chamber and a gene-binding agent capable of binding with a gene and generating a detectable signal being packed in said detection chamber, wherein:
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(1) at least the reaction chamber is imparted with conditions capable of amplifying the gene to be detected;
(2) following the operation of (1), the partition member is unsealed while maintaining the gene detection tool in a sealed state;
(3) through a passage created in the partition member by the unsealing, the solution in the reaction chamber is brought into contact with the gene-binding agent capable of binding the gene in the detection chamber and generating a detectable signal; and
(4) the signal obtained is detected. - View Dependent Claims (13, 14, 15, 16, 17)
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18. A gene detection method characterized by comprising the steps of:
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(1) inserting a test sample potentially containing the gene to be detected and the gene amplification reaction solution through an opening means into the reaction chamber and then closing the opening means to seal the reaction chamber;
(2) imparting conditions permitting amplification of the gene to be detected to the interior of the reaction chamber;
(3) following the elapse of a period of time adequate for amplification of the gene to be detected and while maintaining the gene detection tool comprising the reaction chamber and detection chamber in an overall sealed state, unsealing by an external operation the partition member that is positioned between the reaction chamber and the detection chamber and seals off the reaction chamber from the detection chamber;
(4) causing the reaction solution in the reaction chamber to move into the detection chamber through a passage created by the unsealing;
(5) contacting the gene-binding agent that binds a gene and generates a detectable signal with the reaction solution in the detection chamber; and
(6) detecting the signal obtained.
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Specification