Dna hybrids and environment cleaning system employing dna hybrids

• US 20060051765A1
• Filed: 05/25/2004
• Published: 03/09/2006
• Est. Priority Date: 05/29/2003
• Status: Abandoned Application

• Current Assignee: Canon Ayutthaya Limited (Canon Inc.)
• Original Assignee: Canon Ayutthaya Limited (Canon Inc.)
• Inventors: Sakakibara, Teigo, Zhang, Zuyi, Kotani, Yoshinori, Nishi, Norio
• Application Number:
  US10/535,894
• Publication Number:
  US 20060051765A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 20/06 comprising oxides or hydroxides of metals not provided for in group B01J20/04
• CPC Class Codes: B01J 20/08 comprising aluminium oxide or hydroxide; comprising bauxite
• CPC Class Codes: B01J 20/103 comprising silica
• CPC Class Codes: B01J 20/28061 being in the range 100-500 m2/g
• CPC Class Codes: B01J 20/3078 Thermal treatment, e.g. calcining or pyrolizing
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens
• CPC Class Codes: B01J 20/3293 Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers
• CPC Class Codes: B01J 20/3297 Coatings in the shape of a sheet
• CPC Class Codes: B01J 2220/42 Materials comprising a mixture of inorganic materials materials coated or impregnated on a carrier B01J20/32
• CPC Class Codes: B01J 2220/58 Use in a single column
• CPC Class Codes: C02F 1/286 using natural organic sorbents or derivatives thereof
• CPC Class Codes: C02F 1/288 using composite sorbents, e.g. coated, impregnated, multi-layered
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

A DNA hybrid which comprises a porous oxide matrix and DNA immobilized thereon, and is useful for environmental clean-up, where the hybrid is prepared by removing a dispersion medium from a dispersion of colloidal oxide and DNA.

Claims

1. A DNA hybrid comprising a porous oxide matrix and a DNA immobilized by the matrix, the DNA hybrid prepared by removing a dispersion medium from a dispersion of a colloidal oxide and DNA.

2. The DNA hybrid according to claim 1, wherein the dispersion medium is removed by heating the dispersion at 25°

C. to 200°

C.

3. The DNA hybrid according to claim 1, wherein the DNA content in the hybrid is 0.01 to 15% by mass.

4. The DNA hybrid according to claim 1 or 3, wherein the colloidal oxide is a mixture of a colloidal silica and at least one colloidal oxide of a metal whose valence is 3 or 4.

5. The DNA hybrid according to claim 1, wherein the oxide comprises at least one oxide selected from the group consisting of aluminum oxide, iron oxide, titanium oxide and zirconium oxide.

11. An environmental cleaning system for removing a harmful substance in water comprising a DNA hybrid according to claim 1 and means for bringing the DNA hybrid into contact with a water containing a harmful substance.

12. An environmental cleaning method by removing a harmful substance in a water comprising a step for bringing a DNA hybrid according to claim 1 into contact with a water containing a harmful substance to adsorb the harmful substance by the DNA hybrid.

6. A method for producing a DNA hybrid comprising the steps of:

preparing a dispersion comprising a colloidal oxide, a DNA and a dispersion medium; and

,

removing the dispersion medium from the dispersion to immobilize the DNA in a porous matrix made of the oxide.

7. The method according to claim 6, wherein the dispersion medium is removed by heating the dispersion at 25°

C. to 200°

C.

8. The method according to claim 6, wherein the DNA content of the DNA hybrid is 0.01 to 15% by mass.

9. The method for producing a DNA hybrid according to claim 6 or 8, wherein the colloidal oxide is a mixture of colloidal silica and at least one colloidal oxide of a metal whose valence is 3 or 4.

10. The method according to claim 9, wherein the oxide comprises at least one oxide selected from the group consisting of aluminum oxide, iron oxide, titanium oxide and zirconium oxide.