Methods and compositions for measuring canine BNP and uses thereof

• US 20060051825A1
• Filed: 09/07/2005
• Published: 03/09/2006
• Est. Priority Date: 09/09/2004
• Status: Abandoned Application

• Current Assignee: BIOSITE INCORPORATED (Abbott Laboratories Incorporated)
• Original Assignee: BIOSITE INCORPORATED (Abbott Laboratories Incorporated)
• Inventors: Buechler, Kenneth F., Buechler, Joseph
• Application Number:
  US11/222,494
• Publication Number:
  US 20060051825A1
• Time in Patent Office: Days
• US Class Current: 435/7.930
• CPC Class Codes: C07K 16/26 against hormones ; against hormone releasing or inhibiting factors
• CPC Class Codes: C07K 2317/622 Single chain antibody (scFv)
• CPC Class Codes: G01N 2333/72 for hormones for neuromediators G01N2333/70571
• CPC Class Codes: G01N 2800/325 Heart failure or cardiac arrest, e.g. cardiomyopathy, congestive heart failure
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: G01N 33/6848 Methods of protein analysis involving mass spectrometry
• CPC Class Codes: G01N 33/6854 Immunoglobulins
• CPC Class Codes: G01N 33/6887 from muscle, cartilage or connective tissue
• CPC Class Codes: G01N 33/6893 related to diseases not provided for elsewhere
• CPC Class Codes: G01N 33/74 involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors

Abstract

The present invention describes compositions and methods designed to determine the presence or amount of BNP or fragments thereof in a sample. In particular, the invention provides materials that may be configured to bind canine BNP in a sandwich assay format. The present invention provides, inter alia, assays designed to rapidly and accurately measure BNP-related species in non-human animals.

Claims

1. A method for determining the presence or amount of BNP-related species in a non-human animal, comprising:

performing a sandwich assay on a sample obtained from said non-human animal, wherein said assay is configured to bind canine BNP, and wherein the results of said sandwich assay are indicative of the presence or amount of said BNP-related species in said sample.

2. A method according to claim 1, wherein said sandwich assay comprises contacting the sample with a first antibody immobilized on a solid support, and a second antibody conjugated to a detectable label.

3. A method according to claim 2, wherein one or both of said first and second antibodies are monoclonal antibodies.

4. A method according to claim 1, wherein said assay is configured to distinguish canine BNP from human BNP.

5. A method according to claim 4, wherein said assay is configured to not distinguish canine BNP from BNP native to one or more species selected from the group consisting of sus, felis, and ovis.

6. A method according to claim 1, wherein said non-human animal is a canine.

7. A method according to claim 1, wherein said non-human animal is a feline.

8. A method according to claim 2, wherein one or both of said first or second antibodies exhibit a substantially greater affinity for canine BNP than for human BNP.

9. A method according to claim 1, wherein the assay is a rapid assay.

10. A method according to claim 1, wherein the assay provides a result within about 1 hour of contacting said sample with said first or second antibody.

11. A method according to claim 1, wherein said assay employs nonradioactive detection.

12. A method according to claim 1, wherein said assay employs fluorescent detection.

13. A method according to claim 1, wherein said assaying step comprises performing mass spectrometry.

14. A method according to claim 1, further comprising relating the presence or amount of said BNP-related species in said sample to the diagnosis or prognosis of a cardiovascular condition.

15. A method according to claim 14, wherein said cardiovascular condition is heart failure.

16. A method according to claim 14, wherein said prognosis is death.

17. A monoclonal antibody that binds to canine BNP with a substantially greater affinity for canine BNP than human BNP.

18. A monoclonal antibody according to claim 17, wherein said antibody is a recombinant antibody.

19. A monoclonal antibody according to claim 17, wherein said antibody is insensitive with respect to canine BNP and BNP native to one or more species selected from the group consisting of sus, felis, and ovis.

20. A kit for determining the presence or amount of BNP-related species in a non-human animal, comprising:

a first antibody that binds canine BNP immobilized on a solid phase; and

a second antibody that binds canine BNP conjugated to a detectable label;

each in an amount sufficient to perform at least one sandwich immunoassay on a sample.