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Fluorescent pH detector system and related methods

  • US 20060051874A1
  • Filed: 08/19/2005
  • Published: 03/09/2006
  • Est. Priority Date: 08/19/2004
  • Status: Active Grant
First Claim
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1. A method for measuring the pH of a sample, comprising:

  • (a) irradiating a fluorescent species immobilized on a substrate with excitation light emanating from a probe physically isolated from the fluorescent species immobilized on the substrate, wherein the fluorescent species immobilized on the substrate is in liquid communication with a sample, wherein the excitation light has a wavelength sufficient to effect fluorescent emission from the fluorescent species, wherein the fluorescent species exhibits a first emission intensity at a first emission wavelength and a second emission intensity at a second emission wavelength, the ratio of the first and second emission intensities being dependent on pH; and

    (b) measuring the first and second emission intensities to determined the pH of the sample.

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