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System and apparatus for sequential processing of analytes

  • US 20060051876A1
  • Filed: 08/18/2005
  • Published: 03/09/2006
  • Est. Priority Date: 05/23/1997
  • Status: Active Grant
First Claim
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1. A method for multistep processing of a plurality of analytes, the method comprising:

  • disposing a population of microparticles, of which at least a plurality have an analyte anchored thereto, in a planar array within a flow chamber;

    delivering processing reagents to the flow chamber, such that said analytes are exposed to the processing reagents, for each of a series of processing steps;

    for each said processing step, detecting and recording an optical signal from each microparticle of the plurality, to produce a sequence of optical signals for each microparticle;

    tracking the positions of said microparticles within the array during said processing steps; and

    correlating said sequences of optical signals with said positions.

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