Polypeptides having cellobiohydrolase II activity and polynucleotides encoding same
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Abstract
The present invention relates to polypeptides having cellobiohydrolase II activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.
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Citations
34 Claims
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1-22. -22. (canceled)
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23. A polypeptide having cellobiohydrolase II activity, selected from the group consisting of:
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(a) a polypeptide comprising an amino acid sequence selected from the group consisting of;
an amino acid sequence which has at least 75%, identity with the amino acid sequence shown as amino acids 1 to 477 of SEQ ID NO;
2,an amino acid sequence which has at least 85% identity with the partial amino acid sequence shown as amino acids 1 to 82 of SEQ ID NO;
4,an amino acid sequence which has at least 85% identity with the amino acid sequence shown as amino acids 1 to 420 of SEQ ID NO;
4,an amino acid sequence which has at least 80% identity with the partial amino acid sequence shown as amino acids 1 to 139 of SEQ ID NO;
6,an amino acid sequence which has at least 95% identity with the partial amino acid sequence shown as amino acids 1 to 102 of SEQ ID NO;
8,an amino acid sequence which has at least 85% identity with the partial amino acid sequence shown as amino acids 1 to 144 of SEQ ID NO;
10,an amino acid sequence which has at least 75% identity with the partial amino acid sequence shown as amino acids 1 to 99 of SEQ ID NO;
12,an amino acid sequence which has at least 85% identity with the partial amino acid sequence shown as amino acids 1 to 140 of SEQ ID NO;
14,an amino acid sequence which has at least 75% identity with the partial amino acid sequence shown as amino acids 1 to 109 of SEQ ID NO;
16,an amino acid sequence which has at least 75% identity with the partial amino acid sequence shown as amino acids 1 to 407 of SEQ ID NO;
16,an amino acid sequence which has at least 75% identity with the partial amino acid sequence shown as amino acids 1 to 143 of SEQ ID NO;
18,an amino acid sequence which has at least 70% identity with the partial amino acid sequence shown as amino acids 1 to 71 of SEQ ID NO;
20,an amino acid sequence which has at least 60% identity with the amino acid sequence shown as amino acids 1 to 220 of SEQ ID NO;
22,an amino acid sequence which has at least 65% identity with the amino acid sequence shown as amino acids 1 to 458 of SEQ ID NO;
24, andan amino acid sequence which has at least 70% identity with the amino acid sequence shown as amino acids 1 to 390 of SEQ ID NO;
26,(b) a polypeptide comprising an amino acid sequence selected from the group consisting of;
an amino acid sequence which has at least 75% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Chaetomium thermophilum, an amino acid sequence which has at least 85% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Myceliophtora thermophila, an amino acid sequence which has at least 80% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Melanocarpus albomyces, an amino acid sequence which has at least 80% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Acremonium thermophilum, an amino acid sequence which has at least 95% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Melanocarpus sp., an amino acid sequence which has at least 85% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Thielavia microspora, an amino acid sequence which has at least 80% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Aspergillus fumigatus, an amino acid sequence which has at least 75% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Aspergillus sp., an amino acid sequence which has at least 85% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Thielavia australiensis, an amino acid sequence which has at least 75% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Aspergillus tubingensis, an amino acid sequence which has at least 75% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Gloeophyllum trabeum, an amino acid sequence which has at least 70% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Meripilus giganteus, an amino acid sequence which has at least 60% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Trichophaea saccata, an amino acid sequence which has at least 65% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Stilbella annulata, and an amino acid sequence which has at least 70% identity with the polypeptide encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in Malbrancheae cinnamomea. (c) a polypeptide comprising an amino acid sequence selected from the group consisting of;
an amino acid sequence which has at least 75% identity with the polypeptide encoded by nucleotides 63 to 1493 of SEQ ID NO;
1,an amino acid sequence which has at least 85% identity with the polypeptide encoded by nucleotides 1 to 246 of SEQ ID NO;
3,an amino acid sequence which has at least 80% identity with the polypeptide encoded by nucleotides 1 to 417 of SEQ ID NO;
5,an amino acid sequence which has at least 95% identity with the polypeptide encoded by nucleotides 1 to 306 of SEQ ID NO;
7,an amino acid sequence which has at least 85% identity with the polypeptide encoded by nucleotides 1 to 432 of SEQ ID NO;
9,an amino acid sequence which has at least 75% identity with the polypeptide encoded by nucleotides 1 to 297 of SEQ ID NO;
11,an amino acid sequence which has at least 85% identity with the polypeptide encoded by nucleotides 1 to 420 of SEQ ID NO;
13,an amino acid sequence which has at least 75% identity with the polypeptide encoded by nucleotides 1 to 330 of SEQ ID NO;
15,an amino acid sequence which has at least 75% identity with the polypeptide encoded by nucleotides 1 to 1221 of SEQ ID NO;
15,an amino acid sequence which has at least 75% identity with the polypeptide encoded by nucleotides 1 to 429 of SEQ ID NO;
17,an amino acid sequence which has at least 70% identity with the polypeptide encoded by nucleotides 1 to 213 of SEQ ID NO;
19,an amino acid sequence which has at least 60% identity with the polypeptide encoded by nucleotides 43 to 701 of SEQ ID NO;
21,an amino acid sequence which has at least 65% identity with the polypeptide encoded by nucleotides 21 to 1394 of SEQ ID NO;
23, andan amino acid sequence which has at least 70% identity with the polypeptide encoded by nucleotides 41 to 1210 of SEQ ID NO;
25,(d) a polypeptide which is encoded by a nucleotide sequence which hybridizes under high stringency conditions with a polynucleotide probe selected from the group consisting of;
(i) the complementary strand of the nucleotides selected from the group consisting of;
nucleotides 63 to 1493 of SEQ ID NO;
1,nucleotides 1 to 246 of SEQ ID NO;
3,nucleotides 1 to 1272 of SEQ ID NO;
3,nucleotides 1 to 417 of SEQ ID NO;
5,nucleotides 1 to 306 of SEQ ID NO;
7,nucleotides 1 to 432 of SEQ ID NO;
9,nucleotides 1 to 297 of SEQ ID NO;
11,nucleotides 1 to 420 of SEQ ID NO;
13,nucleotides 1 to 330 of SEQ ID NO;
15,nucleotides 1 to 1221 of SEQ ID NO;
15,nucleotides 1 to 429 of SEQ ID NO;
17,nucleotides 1 to 213 of SEQ ID NO;
19,nucleotides 43 to 701 of SEQ ID NO;
21,nucleotides 21 to 1394 of SEQ ID NO;
23, andnucleotides 41 to 1210 of SEQ ID NO;
25.(ii) the complementary strand of the nucleotides selected from the group consisting of;
nucleotides 63 to 563 of SEQ ID NO;
1,nucleotides 43 to 543 of SEQ ID NO;
21,nucleotides 21 to 521 of SEQ ID NO;
23, andnucleotides 41 to 541 of SEQ ID NO;
25.(iii) the complementary strand of the nucleotides selected from the group consisting of;
nucleotides 63 to 263 of SEQ ID NO;
1,nucleotides 1 to 200 of SEQ ID NO;
3,nucleotides 1 to 1272 of SEQ ID NO;
3,nucleotides 1 to 200 of SEQ ID NO;
5,nucleotides 1 to 200 of SEQ ID NO;
5,nucleotides 1 to 200 of SEQ ID NO;
7,nucleotides 1 to 200 of SEQ ID NO;
9,nucleotides 1 to 200 of SEQ ID NO;
13,nucleotides 1 to 200 of SEQ ID NO;
11,nucleotides 1 to 200 of SEQ ID NO;
13,nucleotides 1 to 200 of SEQ ID NO;
15,nucleotides 1 to 1221 of SEQ ID NO;
15nucleotides 1 to 200 of SEQ ID NO;
17,nucleotides 1 to 200 of SEQ ID NO;
19,nucleotides 43 to 243 of SEQ ID NO;
21,nucleotides 21 to 221 of SEQ ID NO;
23, andnucleotides 41 to 241 of SEQ ID NO;
25.(e) a fragment of (a), (b) or (c) that has cellobiohydrolase II activity. - View Dependent Claims (24, 25, 26, 27, 28, 29, 33, 34)
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30. A polynucleotide comprising a nucleotide sequence which has at least 80% with a nucleotide selected from the group consisting of:
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nucleotides 63 to 1493 of SEQ ID NO;
1,nucleotides 1 to 246 of SEQ ID NO;
3,nucleotides 1 to 1272 of SEQ ID NO;
3,nucleotides 1 to 417 of SEQ ID NO;
5,nucleotides 1 to 306 of SEQ ID NO;
7,nucleotides 1 to 432 of SEQ ID NO;
9,nucleotides 1 to 297 of SEQ ID NO;
11,nucleotides 1 to 420 of SEQ ID NO;
13,nucleotides 1 to 330 of SEQ ID NO;
15,nucleotides 1 to 1221 of SEQ ID NO;
15nucleotides 1 to 429 of SEQ ID NO;
17,nucleotides 1 to 213 of SEQ ID NO;
19,nucleotides 43 to 701 of SEQ ID NO;
21,nucleotides 21 to 1394 of SEQ ID NO;
23, andnucleotides 41 to 1210 of SEQ ID NO;
25.
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31. A polynucleotide having a nucleotide sequence which encodes a polypeptide having cellobiohydrolase II activity, and which hybridizes under high stringency conditions with a polynucleotide probe selected from the group consisting of:
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(i) the complementary strand of the nucleotides selected from the group consisting of;
nucleotides 63 to 1493 of SEQ ID NO;
1,nucleotides 1 to 246 of SEQ ID NO;
3,nucleotides 1 to 1272 of SEQ ID NO;
3,nucleotides 1 to 417 of SEQ ID NO;
5,nucleotides 1 to 306 of SEQ ID NO;
7,nucleotides 1 to 432 of SEQ ID NO;
9,nucleotides 1 to 297 of SEQ ID NO;
11,nucleotides 1 to 420 of SEQ ID NO;
13,nucleotides 1 to 330 of SEQ ID NO;
15,nucleotides 1 to 1221 of SEQ ID NO;
15nucleotides 1 to 429 of SEQ ID NO;
17,nucleotides 1 to 213 of SEQ ID NO;
19,nucleotides 43 to 701 of SEQ ID NO;
21,nucleotides 21 to 1394 of SEQ ID NO;
23, andnucleotides 41 to 1210 of SEQ ID NO;
25.(ii) the complementary strand of the nucleotides selected from the group consisting of;
nucleotides 63 to 563 of SEQ ID NO;
1,nucleotides 43 to 543 of SEQ ID NO;
21,nucleotides 21 to 521 of SEQ ID NO;
23, andnucleotides 41 to 541 of SEQ ID NO;
25.(iii) the complementary strand of the nucleotides selected from the group consisting of;
nucleotides 63 to 263 of SEQ ID NO;
1,nucleotides 1 to 200 of SEQ ID NO;
3,nucleotides 1 to 200 of SEQ ID NO;
5,nucleotides 1 to 200 of SEQ ID NO;
7,nucleotides 1 to 200 of SEQ ID NO;
9,nucleotides 1 to 200 of SEQ ID NO;
11,nucleotides 1 to 200 of SEQ ID NO;
13,nucleotides 1 to 200 of SEQ ID NO;
15,nucleotides 1 to 1221 of SEQ ID NO;
15nucleotides 1 to 200 of SEQ ID NO;
17,nucleotides 1 to 200 of SEQ ID NO;
19,nucleotides 43 to 243 of SEQ ID NO;
21,nucleotides 21 to 221 of SEQ ID NO;
23, andnucleotides 41 to 241 of SEQ ID NO;
25.
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32. A polypeptide having cellobiohydrolase II activity which is encoded by the cellobiohydrolase II encoding part of the nucleotide sequence present in a microorganism selected from the group consisting of:
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a microorganism belonging to the family Chaetomiaceae, a microorganism belonging to the genus Myceliophthora, a microorganism belonging to the species Acremonium thermophilum, a microorganism belonging to the family Chaetomiaceae, a microorganism belonging to the genus Aspergillus, a microorganism belonging to the family Chaetomiaceae, p, a microorganism belonging to the genus Aspergillus, a microorganism belonging to the Polyporales, a microorganism belonging to the Hymenochaetales, pa microorganism belonging to the Pezizomycotina, a microorganism belonging to the species Stilbella annulata, and a microorganism belonging to the species Malbrancheae cinnamomea.
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Specification