Capillary tube printing tips for microarray printing

• US 20060056904A1
• Filed: 10/07/2002
• Published: 03/16/2006
• Est. Priority Date: 10/05/2001
• Status: Abandoned Application

• Current Assignee: Vanderbilt University
• Original Assignee: Vanderbilt University
• Inventors: Haselton, Frederick R, McQuain, Mark K
• Application Number:
  US10/513,803
• Publication Number:
  US 20060056904A1
• Time in Patent Office: Days
• US Class Current: 401/198
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00367 capillary
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00691 using robots
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2300/161 Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 2400/022 droplet contacts the surface of the receptacle
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B01L 3/0262 using touch-off at substrate or container

Abstract

A microarray contact printing is formed from at least one capillary tube. The tip has concentric reservoir and printing capillary tubes, with a first capillary tube (24) and a second capillary tube (22) having an inner bore (26) with an inner diameter larger than an outer diameter of the first capillar tube (24) so that the second capillary tube (22) partially overlaps a proximal end of the first capillary tube (24). The first capillary tube (24) has a contact surface (36) at a distal end. The inner bore of the first capillary tube (24) is adapted for drawing the printing solution retained in the second capillary tube (22) and depositing a drop of a solution on a printing substrate when the contact surface (36) is moved proximate the substrate.

Claims

1. A printing tip for depositing spots of a liquid material on a microarray printing substrate comprising:

a. a first capillary tube having a distal end, a proximal end, and an inner bore, the inner bore having a distal bore opening at the distal end of the first capillary tube, the inner bore having an axial length and inner diameter adapted to receive and retain by capillary force an effective deposition volume of the liquid material;

b. the distal end of the first capillary tube defining an annular contact surface around the distal bore opening, the contact surface and distal bore opening adapted for drawing the liquid material from the inner bore and depositing a drop of the liquid material on the printing substrate when the contact surface is moved proximate the substrate; and

c. gradient means to apply increased surface forces to the liquid material within the tip at a distal end of the tip compared to a proximal end of the tip.

2. The printing tip of claim 1 wherein the gradient means comprises a first capillary tube having an inner bore diameter at the distal end of the tube that is smaller than the inner bore diameter at the proximal end of the tube.

3. The printing tip of claim 1 wherein the gradient means comprises a hydrophilic coating applied to a region of the inner bore proximate the distal end of the tube.

4. The printing tip of claim 1 wherein the contact surface is polished to enhance active wetting of the contact surface by the liquid material.

5. The printing tip of claim 1 wherein the annular contact surface is flat.

6. The printing tip of claim 1 wherein the gradient means comprises a second capillary tube joined to and partially overlapping the distal end of the first capillary tube, the second capillary tube having an inner bore in fluid communication with the inner bore of the first capillary tube, and wherein the inner bore of the second capillary tube is functional to act as a reservoir for the liquid material.

7. The printing tip of claim 6 wherein the second capillary tube is joined to the first capillary tube by an adhesive.

8. The printing tip of claim 7 wherein the adhesive is heat cured.

9. The printing tip of claim 7 wherein the adhesive is photo-cured.

10. The printing tip of claim 1 wherein the first capillary tube has an outer diameter in the range of 15 to 500 microns.

11. The printing tip of claim 10 wherein the inner bore of the first capillary tube has an inner diameter in the range of 5 to 250 microns.

12. The printing tip of claim 10 wherein the inner bore of the first capillary tube has an axial length in the range of 500 to 1500 microns.

13. A tip for depositing a liquid material on a microarray printing substrate comprising:

a. a reservoir section;

b. a contact section having a proximal end and a distal end, the proximal end of the contact section joined to the reservoir section, the contact section further comprising an inner bore extending upward from a bore opening at the distal and of the contact section, the bore in fluid communication with the reservoir section, the distal end of the contact section further defining a contact surface adjacent the bore opening;

c. the reservoir section is adapted to receive and retain by capillary force an effective deposition volume of the liquid material; and

d. the contact surface and bore opening are adapted for depositing a drop of the liquid material when the contact surface is moved proximate the printing substrate.

14. The printing tip of claim 13 wherein the reservoir section comprises a proximal region of the inner bore having an inner diameter the is larger than the inner diameter of the inner bore proximate the contact surface.

15. The printing tip of claim 13 wherein the contact surface is flat.

16. A contact printing tip for printing a spot of a liquid material on a microarray printing substrate comprising:

a. first and second concentric capillary tubes, the second capillary tube having an inner bore with an axial length, and an inner diameter that is larger than an outer diameter of the first capillary tube so that the second capillary tube partially overlaps a proximal end of the first capillary tube;

b. the first capillary tube having an inner bore in fluid communication with the inner bore of the second capillary tube;

c. the first capillary tube further comprising a contact surface at a distal end of the first capillary tube, the contact surface surrounding an opening from the inner bore of the first capillary tube;

d. the inner bore of the second capillary tube having an axial length, an inner diameter, and inner bore surface adapted to receive and retain an amount of the liquid material; and

e. the inner bore of the first capillary tube having an axial length, an inner diameter, and an inner bore surface adapted for drawing the liquid material retained in inner bore of the second capillary tube by capillary action and depositing a drop of the liquid material on the printing substrate when the contact surface is moved proximate the substrate.

17. The printing tip of claim 16 wherein the inner bore of the first capillary tube has an inner diameter in the range of 5 to 250 microns.

18. The printing tip of claim 17 wherein the inner bore of the second capillary tube has an inner diameter in the range of 25 to 4000 microns.

19. The printing tip of claim 18 wherein the inner bore of the first capillary tube has an axial length in the range of 500 to 1500 microns.

20. The printing tip of claim 19 wherein the inner bore of the second capillary tube has an axial length in the range of 500 to 4000 microns.

21. The printing tip of claim 16 wherein the first capillary tube comprises stainless steel.