Electroporation device
First Claim
1. Electroporation device for the permeabilization of cells (C) contained in a substrate (12) comprising signal generating means (3) for generating a stimulating signal (S(t)) applied by means of electrodes (6,7) to the substrate (12) wherein an electric field (E(t)) permeabilizing the cells membranes is induced;
- the device being characterized by comprising;
means for measuring, calculating and monitoring (15,16,23) the instantaneous value of the ration (GT) of current (ie) flowing between said electrodes (6,7) and through the substrate (12) and the voltage (Vp) of the stimulating signal (S(t)) applied to the substrate (12) by means of said electrodes (6,7);
said device further comprising controlling means (100-170) for applying the stimulating signal in a controlled manner according to the waveform of an initial portion of the curve CGT representing the ratio (GT) in successive instants after the beginning of the application of the stimulating signal (S(t)).
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Accused Products
Abstract
Electro-poration device for the permeabilization of cells contained in a substrate comprising a signal generator for generating a stimulating signal applied by means of electrodes to the substrate wherein an electric field permeabilizing the cells membranes is induced. The device calculates and monitors the instantaneous value or the ratio GT between the current flowing through the substrate and the voltage of the stimulating signal applied to the substrate; in particular, the stimulating signal is applied in a modified manner according to the shape of an initial portion of the waveform of a curve CGT representing the value of ratio GT in successive instants after the application of the stimulating signal. The system will achieve permeabilization of cells in substrate with no or minimum damage, allowing to introduce or to extract molecules in or from the electropermeabilized living cells.
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Citations
14 Claims
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1. Electroporation device for the permeabilization of cells (C) contained in a substrate (12) comprising signal generating means (3) for generating a stimulating signal (S(t)) applied by means of electrodes (6,7) to the substrate (12) wherein an electric field (E(t)) permeabilizing the cells membranes is induced;
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the device being characterized by comprising;
means for measuring, calculating and monitoring (15,16,23) the instantaneous value of the ration (GT) of current (ie) flowing between said electrodes (6,7) and through the substrate (12) and the voltage (Vp) of the stimulating signal (S(t)) applied to the substrate (12) by means of said electrodes (6,7);
said device further comprising controlling means (100-170) for applying the stimulating signal in a controlled manner according to the waveform of an initial portion of the curve CGT representing the ratio (GT) in successive instants after the beginning of the application of the stimulating signal (S(t)). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification