Detecting RNAi using SELDI mass spectrometry
First Claim
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1. A method comprising:
- a. expressing polypeptides in a first translation system, wherein the first translation system comprises at least one inhibitory nucleic acid directed toward at least one mRNA; and
b. measuring expression of at least one polypeptide in the translation system by affinity mass spectometry.
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Abstract
The present invention relates to the fields of protein expression and molecular biology. In particular, the present invention includes methods for monitoring the levels of polypeptide inhibition caused by inhibitory nucleic acids.
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Citations
26 Claims
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1. A method comprising:
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a. expressing polypeptides in a first translation system, wherein the first translation system comprises at least one inhibitory nucleic acid directed toward at least one mRNA; and
b. measuring expression of at least one polypeptide in the translation system by affinity mass spectometry. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A kit comprising:
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a. A first expression system capable of expressing at least one inhibitory nucleic acid; and
b. At least one affinity mass spectrometry probe. - View Dependent Claims (23, 24, 25)
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26. A kit comprising:
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a. at least one inhibitory nucleic acid capable of inhibiting expression of at least one expressed protein of a species; and
,b. at least one affinity mass spectrometry probe capable of binding at least one of the expressed proteins.
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Specification