Charcoal stabilization of phenyl phosphates
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Abstract
The invention discloses methods, compositions and kits for stabilizing a solubilized phenyl phosphate, preferably paranitrophenyl phosphate (PNPP), using charcoal. Also disclosed are methods, compositions and kits for recycling solubilized phenyl phosphate, preferably PNPP, that has an absorbance of less than 0.1 when measured at 405 nm due to non-enzymatic hydrolysis.
9 Citations
59 Claims
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1-9. -9. (canceled)
- 10. A kit for phosphatase-phenyl phosphate reactions comprising a phosphatase and a stabilized, colorless solubilized phenyl phosphate comprising phenyl phosphate, a buffer, and charcoal.
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13-42. -42. (canceled)
- 43. A kit for recycling a solubilized phenyl phosphate having an absorbance over 0.1 OD due to non-enzymatic hydrolysis when measured at 400 to 410 nm comprising a solubilized phenyl phosphate which has been colored due to non-enzymatic hydrolysis and a stabilizing amount of charcoal.
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59-1. The kit of claim 11, wherein the solubilized phenyl phosphate comprises approximately 1.5 g/L paranitrophenyl phosphate.
Specification