Method for the spectroscopic determination of the oxygen saturation of blood in the presence of optical disturbance varibles

• US 20060063994A1
• Filed: 04/17/2003
• Published: 03/23/2006
• Est. Priority Date: 04/17/2002
• Status: Active Grant

• Current Assignee: Carl Zeiss Meditec AG (Carl-Zeiss-Stiftung)
• Original Assignee: Carl Zeiss Meditec AG (Carl-Zeiss-Stiftung)
• Inventors: Schweitzer, Dietrich, Hammer, Martin, Thamm, Eike
• Granted Patent: US 7,333,842 B2
• Time in Patent Office: Days
• US Class Current: 600/323
• CPC Class Codes: A61B 5/14551 for measuring blood gases
• CPC Class Codes: A61B 5/14555 specially adapted for the eye fundus

Abstract

The invention is directed to a method for the spectrometric determination of the oxygen saturation of blood in the presence of optical disturbance variables in which transmission measurements and reflection measurements are carried out in at least two wavelengths that are isosbestic for hemoglobin and oxyhemoglobin, and at least one other wavelength at which the extinction of hemoglobin and oxyhemoglobin differ. Corresponding auxiliary functions are defined in the measurement spectrum (M) and in the reference spectra of hemoglobin and oxyhemoglobin, at least two of the measurement values or two of the reference values for the isosbestic wavelengths lying on this auxiliary function. A corrected measurement spectrum (M″) is generated by the two auxiliary functions. The oxygen saturation is determined by comparing the changed data of this corrected measurement spectrum (M″) with the data of the reference spectra at the other wavelength.

Claims

1-3. -3. (canceled)

4. A method for determining the oxygen saturation of blood in the presence of optical disturbance variables, particularly due to a biological tissue surrounding the blood vessel and/or the blood and/or the blood vessel itself comprising the steps of:

generating spectral measurements (M_i) by transmission measurement and reflection measurement in a measurement spectrum at wavelengths that are isosbestic for hemoglobin (Hb) and oxyhemoglobin (HbO₂);

generating at least one other measurement value (M_a) at a wavelength at which the reference values of hemoglobin and oxyhemoglobin differ; and

comparing these measurements with known reference values of the reference spectra of hemoglobin and oxyhemoglobin; and

further comprising the steps of;

a) detecting at least two said spectral measurement values (M_i1, M_i2) at wavelengths (λ

_i1, λ

_i2) that are isosbestic for hemoglobin and oxyhemoglobin and at least the other measurement value (M_a) at a wavelength (λ

_a) at which the reference values of hemoglobin and oxyhemoglobin differ as far as possible in the reference spectra in the measurement spectrum, wherein an auxiliary function (F_H) is generated at least from two of the measurement values (M_i1, M_i2) for isosbestic wavelengths (λ

_i1, λ

_i2);

b) generating a reference function (F_R) in the reference spectra from the reference values (R_i1, R_i2) corresponding to the at least two measurement values (M_i1, M_i2) determined in the measurement spectrum for the same isosbestic wavelengths (λ

_i1, λ

_i2) of hemoglobin and oxyhemoglobin, which reference function (F_R) is of the same type;

c) generating a correction function (F_K) from the auxiliary function (F_H) of the measurement spectrum in which said at least two measurement values (M_i1, M_i2) lie for isosbestic wavelengths (λ

_i1, λ

_i2) and from the reference function (F_R) of the reference spectra in which the at least two reference values (R_i1, R_i2) corresponding to the at least two measurement values (M_i1, M_i2) lie, and generating a corrected auxiliary function (F_Hk) identical to the reference function (F_R) in the reference spectra in a corrected measurement spectrum by this correction function (F_K); and

d) determining the oxygen saturation of the blood from the other measurement value (M_a″

) converted to the corrected auxiliary function (F_Hk) of the corrected measurement spectrum in relation to the reference values for hemoglobin and oxyhemoglobin at this wavelength (λ

_a).

5. The method according to claim 4, including the steps of:

a) logarithmically determining three said spectral measurement values (M_i1, M_i2, M_i3) at wavelengths (λ

_i1, λ

_i2, λ

_i3) that are isosbestic for hemoglobin and oxyhemoglobin and another measurement value (M_a) at a wavelength (λ

_a) at which the reference values of hemoglobin and oxyhemoglobin differ as far as possible in the reference spectra, wherein a linear auxiliary function (F_H) is generated from two logarithmic measurement values (M_i1, M_i2) for isosbestic wavelengths (λ

_i1, λ

_i2);

b) generating a linear reference function (F_R) in the reference spectra from the reference values (R_i1, R_i2) corresponding to the measurement values (M_i1, M_i2) determined in the measurement spectrum for the same isosbestic wavelengths (λ

_i1, λ

_i2) of hemoglobin and oxyhemoglobin;

c) generating a linear correction function (F_K) from the auxiliary function (F_H) of the measurement spectrum and from the reference function (F_R) of the reference spectra, and generating a likewise linear corrected auxiliary function (F_Hk) identical to the linear reference function (F_R) in the reference spectra in the corrected measurement spectrum by this linear correction function (F_K);

d) applying a constant multiplier to the rest of the corrected spectral measurement values, i.e., the third spectral measurement value (M_i3′

) at a wavelength (λ

_i3) that is isosbestic for hemoglobin and oxyhemoglobin and the other measurement value at a wavelength (λ

_a) at which the reference values of hemoglobin and oxyhemoglobin differ as far as possible in the reference spectra, this constant multiplier being determined in such a way that the third spectral measurement value (M_i3′

) of the corrected measurement spectrum that is corrected in this way conforms to the corresponding reference value of the reference spectra; and

e) reading off the oxygen saturation of the blood at the other measurement value (M_a″

) that is converted to the corrected auxiliary function (F_Hk) of the corrected measurement spectrum on a scale from 0 to 1 contained by the reference values for hemoglobin and oxyhemoglobin at this wavelength (λ

_a).

6. The method according to claim 4, wherein, for purposes of a two-dimensional representation of the oxygen saturation of the blood, four monochromatic individual images of the spectral measurement values (M_i, M_a) are generated, and wherein the oxygen saturation is determined according to steps a) to d) for each image point