Methods and device for DNA sequencing using surface enhanced Raman scattering (SERS)
First Claim
1. A method comprising:
- a) sequentially removing nucleotides from one end of at least one nucleic acid molecule;
b) moving the nucleotides through a channel packed with nanoparticles;
c) identifying one or more nucleotides by Raman spectroscopy; and
d) characterizing the nucleic acid.
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Abstract
The methods and apparatus disclosed herein concern nucleic acid characterization by enhanced Raman spectroscopy. In certain embodiments of the invention, exonuclease treatment of the nucleic acids results in the release of nucleotides. The nucleotides may pass from a reaction chamber through a microfluidic channel and enter a nanochannel or microchannel. The nanochannel or microchannel may be packed with nanoparticle aggregates containing hot spots for Raman detection. As the nucleotides pass through the nanoparticle hot spots, they may be detected by Raman spectroscopy. Identification of the sequence of nucleotides released from the nucleic acid is used to characterize the nucleic acid, for example by sequencing or identifying the nucleic acid. Other embodiments of the invention concern apparatus for nucleic acid sequencing.
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Citations
26 Claims
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1. A method comprising:
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a) sequentially removing nucleotides from one end of at least one nucleic acid molecule;
b) moving the nucleotides through a channel packed with nanoparticles;
c) identifying one or more nucleotides by Raman spectroscopy; and
d) characterizing the nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 25)
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17. A method comprising:
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a) preparing a nucleic acid comprising labeled nucleotides;
b) sequentially removing nucleotides from one end of the nucleic acid;
c) moving the nucleotides through a channel packed with nanoparticles;
d) identifying one or more nucleotides by Raman spectroscopy; and
e) characterizing the nucleic acid. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24)
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26-30. -30. (canceled)
Specification