Multi-array, multi-specific electrochemiluminescence testing
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Abstract
Materials and methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use multiply specific testing procedures.
70 Citations
139 Claims
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1-108. -108. (canceled)
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109. A method for detecting or measuring an analyte of interest in a sample in an electrochemiluminescence binding assay comprising:
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(a) adding a liquid sample containing an analyte of interest to an assay cassette that comprises (i) an assay electrode; and
(ii) a dry reagent composition comprising at least one member of the group consisting of (1) a labeled binding reagent comprising an electrochemiluminescent label and (2) an electrochemiluminescence coreactant;
(b) reconstituting said dry reagent composition in said liquid sample;
(c) contacting said electrode with said reconstituted dry reagent composition;
(d) applying a voltage waveform effective to trigger electrochemiluminescence at said electrode; and
(e) detecting or measuring electrochemiluminescence;
wherein said detected or measured electrochemiluminescence correlates to the presence or amount of said analyte in said sample. - View Dependent Claims (110, 112, 113, 115, 116, 117, 118, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133)
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134. A cassette for detecting or measuring an analyte of interest in a sample in an electrochemiluminescence binding assay comprising:
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(a) an assay electrode; and
(b) a dry reagent composition comprising at least one member of the group consisting of i) a labeled binding reagent comprising an electrochemiluminescent label and ii) an electrochemiluminescence coreactant. - View Dependent Claims (135, 136, 137, 138, 139)
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Specification