Enzyme-based device for environmental monitoring
First Claim
1. An enzyme-based environmental monitoring device for detecting a target analyte comprising:
- an immobilized enzyme;
a mechanism for delivering an environmental sample to said immobilized enzyme, wherein said environmental sample optionally contains a target analyte;
a mechanism for delivering an enzyme substrate compound and optionally a pH sensitive dye to said immobilized enzyme; and
one or more sensors for detecting the level of enzyme activity of said immobilized enzyme and presence, if any, of said target analyte, wherein said target analyte acts as an enzyme inhibitor in the presence of said immobilized enzyme and said enzyme substrate compound.
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Accused Products
Abstract
A sensor for the intermittent or continuous detection of the presence of at least one analyte in an environmental sample includes at least one enzyme that is selected to either (i) catalyze a reaction of the analyte to chemically convert the analyte to a product compound or (ii) be inhibited by the analyte in the presence of a substrate compound. The sensor also includes at least one sensor for monitoring or at least one indicator compound selected to produce a measurable change of state as a result of the interaction of the analyte and the enzyme. Optionally, each of the enzyme and the indicator compound are incorporated within a single polymer.
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Citations
29 Claims
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1. An enzyme-based environmental monitoring device for detecting a target analyte comprising:
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an immobilized enzyme;
a mechanism for delivering an environmental sample to said immobilized enzyme, wherein said environmental sample optionally contains a target analyte;
a mechanism for delivering an enzyme substrate compound and optionally a pH sensitive dye to said immobilized enzyme; and
one or more sensors for detecting the level of enzyme activity of said immobilized enzyme and presence, if any, of said target analyte, wherein said target analyte acts as an enzyme inhibitor in the presence of said immobilized enzyme and said enzyme substrate compound. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 23, 24)
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22. An enzyme-based environmental monitoring device for detecting contamination in a sample of water or air comprising:
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an immobilized enzyme covalently bonded with a polymer to form an immobilization matrix;
a first pump or fan for continuously delivering a stream of environmental water or air to said immobilization matrix;
a second pump for delivering an enzyme substrate compound and optionally a pH sensitive dye compound to said stream of water or air prior to its delivery to said immobilization matrix; and
one or more sensors for detecting the level of enzyme activity at said immobilization matrix. - View Dependent Claims (25)
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26. An enzyme-based environmental monitoring device for detecting a target analyte comprising:
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a first reaction system including a first enzyme and a substrate for the first enzyme, a mechanism for delivering an environmental sample to the device either intermittently or continuously, wherein said environmental sample optionally contains the analyte, wherein the analyte inhibits the first enzyme; and
at least a second reaction system that reacts to produce a first detectable state only when the first enzyme is inhibited. - View Dependent Claims (27, 28)
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29. An enzyme-based environmental monitoring device for detecting a target analyte comprising:
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at least one enzyme that is selected to catalyze a reaction of the analyte to chemically convert the analyte to a product compound, a mechanism for delivering an environmental sample to the device either intermittently or continuously, wherein the environmental sample optionally contains said analyte, and at least one sensor selected to monitor or at least one indicator compound to produce a measurable change of state as a result of the interaction of the analyte and the enzyme.
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Specification