Measuring device and measuring method for detecting analytes
First Claim
1. A device for the detection and/or determination of the concentration of at least one analyte present in a liquid medium, consisting of a) a strip-shaped carrier (20) having two longitudinal edges (24), to which carrier (20) a printed multiple electrode set consisting of at least one reference electrode (30) and at least one working electrode (40) is applied and b) ligands which are suitable for binding a biologically active specific binding molecule or the analyte, wherein the device a) comprises a rigid reaction and detection chamber (50) adapted for continous flow of the medium along said strip-shaped carrier (20), said reaction and detection chamber being formed by an elongated support (52) applied to said longitudinal edges (24) of said carrier (20) having a cover (54) disposed on said support (52) and that b) a liquid absorption element (60) is disposed downstream of said reaction and detection chamber (50).
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Accused Products
Abstract
A measuring device for the detection and determination of the concentration of at least one analyte in a liquid medium consists of a polymeric carrier having biologically active specific binding molecules dried on its upstream portion. A multiple electrode set consisting of at least one reference electrode and at least one working electrode is located thereon in a downstream region. The working electrode is provided with biologically active specific binding molecules. The carrier has a sample application zone, which is adjoined, in the downstream direction, by a non-deformable, through-flow reaction and detection chamber, a variably dimensioned interstice, and a liquid absorption element. For a qualitative and quantitative analyte determination, two liquid media are applied in succession to the sample application zone. While the first can contain the wanted analyte, the second provides for the formation of an electrical signal.
26 Citations
54 Claims
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1. A device for the detection and/or determination of the concentration of at least one analyte present in a liquid medium, consisting of
a) a strip-shaped carrier (20) having two longitudinal edges (24), to which carrier (20) a printed multiple electrode set consisting of at least one reference electrode (30) and at least one working electrode (40) is applied and b) ligands which are suitable for binding a biologically active specific binding molecule or the analyte, wherein the device a) comprises a rigid reaction and detection chamber (50) adapted for continous flow of the medium along said strip-shaped carrier (20), said reaction and detection chamber being formed by an elongated support (52) applied to said longitudinal edges (24) of said carrier (20) having a cover (54) disposed on said support (52) and that b) a liquid absorption element (60) is disposed downstream of said reaction and detection chamber (50).
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43. A method for the production of a device (10) for the detection and determination of the concentration of at least one analyte dissolved in a liquid medium, wherein
a) an electrode material is worked to a paste and this paste is applied to a carrier (20) in such a way that a multiple electrode set consisting of at least one working electrode (40) and at least one reference electrode (30) and adjoining conductive tracks (42) and contacts (44) is formed, b) said carrier (20) provided with said multiple electrode set is dried at elevated temperature, c) ligands and/or biologically active specific binding molecules and/or catalytically active proteins are applied to said carrier (20), namely in said region of said multiple electrode set or in a region which is disposed upstream of said multiple electrode set and d) an elongated support (52), a cover (54), and a liquid absorption element (60) are fixed to the carrier (20) thus obtained such that said cover (54) and said carrier (20) are spaced from one another.
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48. A method for the detection and determination of the concentration of at least one analyte dissolved in a liquid medium using a device (10) which consists of at least one working electrode (40) and at least one reference electrode (30, wherein
a) a definable amount of said liquid medium is applied to a sample application zone (22) of said device (10), b) a specified period of time is allowed to elapse in order to allow interaction of said analyte(s) with ligands and/or biologically active specific binding molecules and/or catalytically active proteins to take place, c) a solution is applied to the sample application zone (22), which contains at least one reaction-specific substrate for a catalytically active protein or a number of catalytically active proteins, d) said device (10) is connected to an electrical measuring apparatus, and e) after an incubation time, an electrical signal is read off on said measuring apparatus connected to said device (10) for each analyte to be determined and a corresponding concentration of the respective analyte is assigned to this signal with the aid of a calibration curve.
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53. (canceled)
Specification