Neural progenitor cells derived from embryonic stem cells
First Claim
1. An enriched preparation of human undifferentiated embryonic stem cells wherein said cells are capable of proliferation in vitro and differentiation to neural progenitor cells, neuron cells and/or glial cells.
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Abstract
The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation and production of differentiated cells. In particular it relates to the production of human ES cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells such as neural progenitor cells capable of giving rise to mature somatic cells including neural cells and/or glial cells and uses thereof. This invention provides methods that generate in vitro and in vivo models of controlled differentiation of ES cells towards the neural lineage. The model, and cells that are generated along the pathway of neural differentiation may be used for: the study of the cellular and molecular biology of human neural development, discovery of genes, growth factors, and differentiation factors that play a role in neural differentiation and regeneration, drug discovery and the development of screening assays for teratogenic, toxic and neuroprotective effects.
38 Citations
73 Claims
- 1. An enriched preparation of human undifferentiated embryonic stem cells wherein said cells are capable of proliferation in vitro and differentiation to neural progenitor cells, neuron cells and/or glial cells.
- 4. An undifferentiated human embryonic stem cell wherein the cell is capable of proliferation in vitro and differentiation to neural progenitor cells, neuron cells and/or glial cells and is immunoreactive with markers for human pluripotent stem cells including SSEA-4, GCTM-2 antigen, and TRA 1-60.
- 8. A differentiated committed human progenitor cell line capable of differentiation and propagation into mature neurons and/or glial cells said cell line derived from undifferentiated human embryonic stem cells.
- 11. A neural progenitor cell differentiated in vitro from an undifferentiated human embryonic stem cell.
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29. A method of preparing undifferentiated human embryonic stem cells for differentiation into neural progenitor cells, said method including:
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obtaining an in vitro fertilised human embryo and growing the embryo to a blastocyst stage of development;
removing inner cells mass (ICM) cells from the embryo;
culturing ICM cells under conditions which do not induce extraembryonic differentiation and cell death, and promote proliferation of undifferentiated stem cells; and
recovering the stem cells. - View Dependent Claims (30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 60, 61, 62, 63, 64)
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40. A method of inducing somatic differentiation of stem cells in vitro into progenitor cells said method comprising:
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obtaining undifferentiated embryonic stem cells; and
providing a differentiating signal under conditions which are non-permissive for stem cell renewal, do not kill cells and/or induces unidirectional differentiation toward extraembryonic lineages. - View Dependent Claims (41, 42, 43, 44, 45, 65, 66, 67, 68, 69, 70, 71, 72, 73)
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46. A method of inducing somatic cells from embryonic stem cell derived somatic progenitors, said method comprising:
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obtaining a source of embryonic stem cell derived somatic progenitors;
culturing the progenitor cells on an adhesive substrate; and
inducing the cells to differentiate to somatic cells under conditions which favour somatic differentiation. - View Dependent Claims (47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59)
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Specification