Compartmentalised combinatorial chemistry by microfluidic control
First Claim
1. A method for preparing a repertoire of compounds comprising the steps of:
- (a) compartmentalising two or more sets of primary compounds into microcapsules;
such that a proportion of the microcapsules contains multiple copies of one or more compounds representative of each of said sets, and wherein said one or more compounds form a subset of the set of primary compounds;
(b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets;
wherein one or more of steps (a) and (b) are performed under microfluidic control.
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Abstract
The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalising two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalised into the microcapsules.
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Citations
112 Claims
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1. A method for preparing a repertoire of compounds comprising the steps of:
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(a) compartmentalising two or more sets of primary compounds into microcapsules;
such that a proportion of the microcapsules contains multiple copies of one or more compounds representative of each of said sets, and wherein said one or more compounds form a subset of the set of primary compounds;
(b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets;
wherein one or more of steps (a) and (b) are performed under microfluidic control. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 101, 102, 104, 105, 106, 107, 108, 109, 110, 111, 112)
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14. A method for identifying primary compounds which react together to form secondary compounds capable of binding to or modulating the activity of a target, comprising the steps of:
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(a) compartmentalising two or more sets of primary compounds into microcapsules;
such that a proportion of the microcapsules contains two or more compounds;
(b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; and
(c) identifying subsets of primary compounds which react to form secondary compounds which bind to or modulate the activity of the target;
wherein one or more of steps (a) and (b) and (c) are performed under microfluidic control. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 86, 87, 88, 89, 100, 103)
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15. A method for synthesising compounds with enhanced ability to bind to or modulate the activity of the target, comprising the steps of:
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(a) compartmentalising into microcapsules subsets of primary compounds identified in step (c) of the second aspect of the invention and, optionally, compartmentalising additional sets of primary compounds;
(b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; and
(c) identifying subsets of primary compounds which react to form secondary compounds which bind to or modulate the activity of the target;
wherein one or more of steps (a) and (b) and (c) are performed under microfluidic control. - View Dependent Claims (16)
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17. A method for identifying individual compounds which bind to or modulate the activity of the target, comprising the steps of:
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(a) compartmentalising into microcapsules a primary compound identified in step (c) of the second or third aspect of the invention and additional sets of primary compounds;
(b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; and
(c) identifying subsets of primary compounds which react to form secondary compounds which bind to or modulate the activity of the target;
wherein one or more of steps (a) and (b) and (c) are performed under microfluidic control.
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Specification