Surface assembly for immobilizing DNA capture probes in genetic assays using enzymatic reactions to generate signal in optical bio-discs and methods relating thereto

• US 20060078935A1
• Filed: 11/18/2005
• Published: 04/13/2006
• Est. Priority Date: 05/18/2001
• Status: Abandoned Application

• Current Assignee: Vindur Technologies, Inc.
• Original Assignee: Vindur Technologies, Inc.
• Inventors: Gordon, John Francis, Valencia, Ramon Magpantay, Werner, Martin Elisabeth
• Application Number:
  US11/282,211
• Publication Number:
  US 20060078935A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00274 Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00536 in the shape of disks
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00578 electrophoric
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/00695 : Synthesis control routines, e.g. using computer programs
• CPC Class Codes: B01J 2219/00702 : Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00707 : separated from the reactor apparatus
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00725 : Peptides
• CPC Class Codes: B01L 2300/0806 : Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2400/0409 : centrifugal forces
• CPC Class Codes: B01L 3/5025 : for parallel transport of multiple samples
• CPC Class Codes: B01L 3/5027 : by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 : characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502753 : characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: B01L 3/545 : for laboratory containers
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 50/14 : Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 35/00069 : whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
• CPC Class Codes: Y10S 977/924 : using nanostructure as support of dna analysis

Abstract

The invention relates to methods and systems for use of bio-discs in quantifying an amount or concentration of one or more substances that are present in a biological sample. The system includes an optical disc having one or more samples deposited thereon, an optical element configured to emit and divert radiation onto the samples and components for measuring spectral change of the compound and determining an amount or concentration of one or more target substances. The bio-disc is prepared using a method including providing a membrane that is or can be dimensional to fit within a channel of the bio-disc, applying a reagent on the membrane which is configured to allow the reagent to be released from the membrane into a solution in contact with the membrane, and depositing the membrane in a channel of the bio-disc.

Claims

1. A method of preparing a bio-disc having at least one channel, the method comprising:

providing a membrane that is or can be dimensioned to fit within the at least one channel of the bio-disc;

applying one or more reagents on the membrane, wherein the membrane is configured to allow the one or more reagents to be released from the membrane into a solution placed in contact with the one or more reagents on the membrane; and

depositing the membrane in one of the at least one channels of the bio-disc.

2. The method of claim 1, wherein the bio-disc comprises a semi-reflective layer having a thickness of less than about 400 Å

.

3. The method of claim 1, wherein the bio-disc comprises a semi-reflective layer having a thickness of between about 100 and 300 Å

.

4. The method of claim 1, wherein the bio-disc comprises a plurality of channels and a plurality of membranes are deposited in the plurality of channels.

5. The method of claim 1, wherein the reagents on the membrane are allowed to dry before the depositing step is performed.

6. The method of claim 1, wherein the one or more reagents comprise one or more enzymes.

7. The method of claim 1, wherein providing a membrane comprises providing a membrane that is or can be dimensioned to fit within a side chamber of the bio-disc that is in fluid communication with the at least one channel.

8. The method of claim 1, wherein the membrane is a bibulous hydrophilic material.

9. The method of claim 1, wherein the membrane comprises hydrophilic polyethersulfone.

10. The method of claim 1, wherein the applying is performed using a pipette.

11. An apparatus for quantifying an optical density change in calorimetric assays, the apparatus comprising:

an optical disc having one or more compounds deposited thereon, wherein the one or more compounds change one or more spectral characteristics in the presence of a target substance so that a spectral change by each of the one or more compounds is a function of a concentration of the target substance brought into contact with each of the one or more compounds;

an optical element configured to emit and direct radiation so that the radiation is incident on the compounds;

a detector configured to measure an indication of the spectral change for each of the one or more compounds;

a computing device responsive to the detector and configured to determine an amount or concentration of one or more target substances.

12. A method of quantifying an amount of one or more analytes present in a biological sample, the method comprising:

providing an optical disc having one or more reagents located on or in one or more analysis zones of the optical disc;

introducing a sample onto or into the optical disc so that the sample contacts the one or more reagents on the optical disc;

incubating the optical disc for a period of time;

quantifying a spectral change in at least one portion of the disc resulting from introduction of the sample; and

determining an amount of the one or more analytes present in the sample based upon results from the quantifying step.

13. The method of claim 12, wherein the sample comprises one or more of a body fluid sample, an agricultural product sample, a food sample, a waste product sample, and an environmental sample.

14. A method of quantifying an amount of one or more analytes present in a biological sample, the method comprising:

depositing one or more reagents onto respective one or more analysis zones on an optical disc;

applying a sample onto the optical disc so that the one or more reagents is brought into contact with the sample;

incubating the optical disc for a period of time;

emitting radiation having a known wavelength so that the radiation is incident upon the sample;

quantifying an amount of radiation transmitted through the sample in contact with each of the one or more reagents; and

determining an amount of the one or more analytes present in sample based upon results from the quantifying step.