Capture and detection of microbes by membrane methods
4 Assignments
0 Petitions
Accused Products
Abstract
Methods and systems for detecting the presence of analytes using a membrane based detection system are described. A fluid sample is passed through a membrane based detection system (100). Particulate analytes (e.g., microbes) are captured by the membrane (110). Detection and analysis techniques may be applied to determine the identity and quantity of the captured analytes.
-
Citations
94 Claims
-
1-49. -49. (canceled)
-
50. A method of analyzing an analyte collected on a membrane comprising:
-
passing a fluid sample across a membrane, wherein the fluid sample comprises an analyte that is at least partially retained by the membrane;
adding a visualization agent to material collected on the membrane when the fluid sample is passed across the membrane;
collecting an image of the collected material using white light, at a first wavelength of light, a second wavelength of light, and a third wavelength of light, wherein the analyte comprises a color corresponding to the first wavelength of light;
forming a first mask corresponding to an image of the collected material at the second wavelength of light;
forming a second mask corresponding to an image of the collected material at the third wavelength of light;
subtracting the first mask and the second mask from the image of the collected material in white light. - View Dependent Claims (51, 52, 53, 54, 81, 82, 83, 84, 85, 86, 87, 88, 89, 94)
-
-
55-68. -68. (canceled)
-
69. A method of sensing an analyte in a fluid comprising:
-
passing the fluid across a porous membrane configured to capture the analyte on the porous membrane;
applying a visualization agent to the particles on the porous membrane;
detecting an image of matter captured on the porous membrane with a detector at a plurality of wavelengths of light;
detecting an image of matter captured on the porous membrane at a specific wavelength of light, wherein the specific wavelength of light represents light that is not indicative of the presence of the analyte. - View Dependent Claims (90, 91, 92, 93)
-
-
70-80. -80. (canceled)
Specification