Use of spatiotemporal response behavior in sensor arrays to detect analytes in fluids

• US 20060088445A1
• Filed: 02/08/2005
• Published: 04/27/2006
• Est. Priority Date: 05/10/1999
• Status: Active Grant

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Lewis, Nathan S., Freund, Michael S., Briglin, Shawn M.
• Granted Patent: US 7,189,353 B2
• Time in Patent Office: Days
• US Class Current: 422/83
• CPC Class Codes: G01N 27/126 comprising organic polymers
• CPC Class Codes: G01N 33/0031 comprising two or more sensors, e.g. a sensor array electrochemical electrode arrays G01N27/27
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/184 Only hydrogen sulfide

Abstract

Methods, systems and sensor arrays are provided implementing techniques for detecting an analyte in a fluid. The techniques include providing a sensor array including at least a first sensor and a second sensor in an arrangement having a defined fluid flow path, exposing the sensor array to a fluid including an analyte by introducing the fluid along the fluid flow path, measuring a response for the first sensor and the second sensor, and detecting the presence of the analyte in the fluid based on a spatio-temporal difference between the responses for the first and second sensors.

Claims

1. A system for identifying an analyte in a fluid, comprising:

a fluid flow chamber having a first end and a second end;

a sensor array including at least a first sensor and a second sensor in an arrangement in the fluid flow chamber such that at least the first sensor is proximal to the first end of the flow chamber and at lest the second sensor is proximal to the second end of the flow chamber thereby having a defined fluid flow path, wherein a fluid moving in the fluid flow chamber contacts the at least two sensors at different times, each of the first and second sensors comprising a sensing area comprising regions of a conductive material and an organic material;

a measuring apparatus coupled to the sensor array, the measuring apparatus being configured to detect a response from the firs sensor and the second sensor upon exposure of the sensor array to the fluid; and

a computer configured to generate data indicating the analyte in the fluid based on a spatio-temporal difference between the responses for the first and second sensors and identifying the analyte based upon the spatio-temporal difference.

2. The system of claim 1, wherein the data includes a spatio-temporal response profile derived from the spatio-temporal difference between the responses for the at least first and second sensors.

3. The system of claim 2, wherein the spatio-temporal response profile is derived from time information indicating the dependence of sensor response on time.

4. The system of claim 3, wherein the first sensor occupies a first position in the arrangement and the second sensor occupies a second position in the arrangement, such that the response of the second sensor is delayed in time with respect to the response of the first sensor upon exposure of the sensor array to the fluid.

5. The system of claim 3, wherein the first sensor occupies a first position in the arrangement and the second sensor occupies a second position in the arrangement, such that the response of the second sensor is changed in amplitude with respect to the response of the first sensor upon exposure of the sensor array to the fluid.

6. The system of claim 5, wherein the first sensor includes a sensing material;

and the response of the first sensor is greater than the response of the second sensor for an analyte having a high affinity for the sensing material.

7. The system of claim 4, wherein the first and second sensors are selected and arranged to provide a first delay between the response of the first sensor and the response of the second sensor upon exposure of the sensor array to a fluid including a first analyte and a second delay between the response of the first sensor and the response of the second sensor upon exposure of the sensor array to a fluid including a second analyte.

8. The system of claim 7, wherein:

the measuring apparatus is configured to measure the delay between the response of the first sensor and the response of the second sensor; and

the spatio-temporal difference between the responses for the first and second sensors is derived from the delay.

9. The system of claim 8, wherein the computer is configured to characterize the analyte based on the spatio-temporal difference between the responses.

10. The system of claim 2, further comprising:

a flow controller to introduce the fluid to the sensor array at a varying flow rate.

11. The system of claim 10 wherein the computer is configured to generate flow information indicating the dependence of sensor response on flow rate.

12. The system of claim 2, wherein the sensor array includes a plurality of cross-reactive sensors.

13. The system of claim 2, wherein the sensor array further comprises a plurality of sensors selected from the group including surface acoustic wave-sensors, quartz crystal resonators, metal oxide sensors, dye-coated fiber optic sensors, dye-impregnated bead arrays, micromachined cantilever arrays, chemically-sensitive resistor or capacitor films, metal-oxide-semiconductor field effect transistors, and bulk organic conducting polymeric sensors.

14. The system of claim 2, wherein the first and second sensors comprise composites having regions of a conducting material and regions of an insulating organic material.

15. The system of claim 2, wherein the first and second sensors comprise composites having regions of a conducting material and regions of a conducting organic material.

16. The system of claim 2, wherein the computer is configured to generate a digital representation of the analyte based at least in part on the responses of the first and second sensors.

17. The system of claim 16, further comprising:

a communications port coupled to the computer for communicating the digital representation of the analyte to a remote location for analysis.

18. The system of claim 1, wherein the organic material is an insulator.

19. The system of claim 1, wherein the organic material is a conductor.

20. The system of claim 1, wherein the conductive material is carbon black.